Clinical Significance Of Detection Of PAC-1and CD62P In Patients With Acute Exacerbation F Chronic Obstructive Pulmonary Disease

Objectives：In this paper,to evaluate platelet activation marker in acute exacerbation ofchronic obstructive pulmonary diseases（AECOPD），to study the role of plateletactivation markers（CD62P、 PAC-1） in chronic obstructive pulmonary diseases（COPD）．In order to guide the doctor to take measures as soon as possible and providethe guidance for the observation of anti-platelet therapy efficacy．So as to reducecomplications and improve prognosis and reduce the mortality of COPD.Methods：1.Study subjects：We collect30cases of patients who are all diagnosed with acuteexacerbation of chronic obstructive pulmonary diseases at department of respiratory inYanan university affiliated hospital from January2012to January2013.There weretwenty-four males and six females, with an average age of68.40±10.98years (range:50to82years).Besides，30cases of chronic obstructive pulmonary diseases in stableperiod，there were fifteen males and fifteen females, with an average age of62.50±3.56years (range:59to76years).Diagnosis accord with the “Global strategy for thediagnosis,management,and prevention of chronic obstructive pulmonary disease(Revised2011)”.30cases of healthy patients，whose physical examinations arenormal,there were fifteen males and fifteen females, with an average age of51.6±8.15years (range:40to62years).There were no significant differences between the twogroups in gender and age.In all experimental cases did not receive2weeks ofAnti-platelet and anticoagulant drugs.And the medical history of basic diseases thataffect platelet disease is rejected. 1.The specific experiments：①.We detect the expressions of PAC-1and CD62P in theperipheral blood among the acute exacerbation of chronic obstructive pulmonarydiseases，chronic obstructive pulmonary diseases in stable period by FCM.And we selectthe PAC-1and CD62P in peripheral blood of the normal people as a contrast.②. Wedetect PO2and PCO2in the arterial blood in acute exacerbation of chronic obstructivepulmonary diseases with arterial blood gas analyzer.Results：1.There is no significant difference in statistics between patients with acute exacerbationof chronic obstructive pulmonary diseases and chronic obstructive pulmonary diseases instable period（age，gender）.2. Expression levels of CD62P in the three groups was （7.03±5.50）%、（4.30±0.35）%、（3.44±2.05）%，the peripheral blood CD62P expression in patients with acuteexacerbation of chronic obstructive pulmonary diseases is higher than chronic obstructivepulmonary diseases in stable period，normal control group，（P<0.05）. the peripheralblood CD62P expression in patients with chronic obstructive pulmonary diseases instable period is higher than normal control group，（P<0.05）.Expression levels of PAC-1in the three groups wa（s28.77±10.54）%、（12.10±5.59）%、（1.20±0.89）%，the peripheralblood PAC-1，expression in patients with acute exacerbation of chronic obstructivepulmonary diseases is higher than chronic obstructive pulmonary diseases in stableperiod，normal control group，（P<0.05）. the peripheral blood PAC-1expression inpatients with chronic obstructive pulmonary diseases in stable period is higher thannormal control group，（P<0.05）.3.We use the Spearman rank correlation test to test the relations between PAC-1andCD62P in acute exacerbation of chronic obstructive pulmonary diseases.The result shows:the PAC-1in acute exacerbation of chronic obstructive pulmonary diseases has positivecorrelation with CD62P（r＝0.79,P<0.05）.4. We use the Spearman rank correlation test to test the relations between artery carbondioxide pressure (PaCO2) and PAC-1、 CD62P in acute exacerbation of chronic obstructive pulmonary diseases. The result shows: the artery carbon dioxide pressure(PaCO2) in acute exacerbation of chronic obstructive pulmonary diseases has positivecorrelation with PAC-1、CD62P（r＝0.71,P<0.05）、（r＝0.86,P<0.05）.5. We use the Spearman rank correlation test to test the relations between artery oxygenpressure (PaO2) and PAC-1、CD62P in acute exacerbation of chronic obstructivepulmonary diseases. The result shows: the artery oxygen pressure (PaO2) in acuteexacerbation of chronic obstructive pulmonary diseases has negative correlation withPAC-1、CD62P（r＝-0.84,P<0.05）、（r＝-0.69,P<0.05）.Conclusion：1. Activated platelets exist in patients with chronic obstructive pulmonary diseases inexacerbation and stable stage，especially complicated with AECOPD.We should takeappropriate measures，it can reduce the risk of thromboembolism in patients withAECOPD.2. The PAC-1in acute exacerbation of chronic obstructive pulmonary diseases haspositive correlation with CD62P. It is suggested that there is synergism between PAC-1and CD62P as the markers of platelet activation in AECOPD.3. The artery carbon dioxide pressure (PaCO2) in acute exacerbation of chronicobstructive pulmonary diseases has positive correlation with PAC-1、CD62P. The arteryoxygen pressure (PaO2) in acute exacerbation of chronic obstructive pulmonary diseaseshas negative correlation with PAC-1、CD62P.Its empirical results indicate that there existinteractions between platelet activation and hypoxia,the retention of carbon dioxide.