| ObjectiveThis thesis aims to measure the levels of tumor necrosis factor-α (TNF-α) andadiponectin (APN) in the blood, and to analyze briefly the impact of the levels ofTNF-a) and APN on coronary heart diseases and the relationship between the both,for providing the method and basis for the early prediction and intervention incardiovascular vicious incident, thereby reducing the mortality rate of cardiovascularevents.MethodsThe subjects were divided into four groups——control group, angina pectorisgroup(AP), myocardial infarction(MI) and myocardial infarction combinedcarotid/renal/cerebral arteriosclerosis group (MIC),of which AP were selected fromthe medical section in the Qinghai University Affiliated Hospital date from October2011to October2012, while the three case groups selected from the CardiovascularDepartment in the same period. TNF-a and APN were measured by enzyme-linkedimmunosorbent assay (ELESA). The SAS version9.2was used to analyze the data.The multivariate analysis of variance was applied to compare the quantitative indicators differences among groups, while the univariate analysis of variance wasused to compare the individual quantitative indicator differences among groups, usingthe SNK test to compare multiple comparisons. All statistical tests methods inspectionlevel α=0.05.Results1. Case group had80subjects, of which AP had31cases, in which51.61%were male, while48.39%were female, and the mean age was63.61±8.19years; MIhad29cases, in which male62.07%, while female37.93%, and mean age66.66±8.64; MIC had20cases, in which male60.00%, while female40.00%, andmean age63.95±11.10; Control group had32cases, in which male62.50%, whilefemale37.50%, and mean age65.13±8.75;2. TG (F=16.33, P <0.0001) and HDL (F=9.11, P <0.0001) were statisticallysignificant differences among the four groups. For the TG, except no statisticallysignificant differences in MI and MIC, other groups were statistically significantdifferences. The descending order of levels of TG were MIC (3.40±1.11), MI (2.60±1.07), AP (1.97±1.01) and control group (1.46±0.97). For the HDL, except nostatistically significant differences in AP and MI, other groups were statisticallysignificant differences. The descending order of levels of TG were control group(1.36±0.29), AP (1.19±0.29), MI (1.06±0.25) and MIC (1.01±0.24).3. Subjects’ levels of TNF-a from high to low in order MIC (3.34±0.92), MI(2.72±0.86), AP (1.63±0.54) and the control group (0.94±0.39), each of whichwere significantly different. The descending order of levels of APN were controlgroup (10.71±2.51), AP (4.95±1.72), MI(3.87±1.60), MIC (2.86±1.24), apartfrom the latter two groups was no statistically significant differences, other groupswere statistically significant differences. TNF-a and APN was negatively correlated（r=-0.85，P<0.001）. For the CRP, apart from MI and MIC, other groups werestatistically significant differences. The descending order of levels of CRP wereMIC (16.85±5.38), MI (16.71±5.53), AP (10.05±3.90) and control group (6.64±2.68).4. It was statistically significant different for the Smoking and the non-smokingproportion among the four groups （211.18,P=0.0108）. Multiple test resultsshowed statistically significant differences in MIC and AP/The control group （211.18,P=0.0008）, the smoking proportion of MIC (60.00%) were higherthan AP(19.35%) and The control group (21.88%).ConclusionsTNF-a is a risk factor for coronary heart disease, direct impacting on the severityof the disease, while APN has a protective effect on the disease, both of which play aantagonistic role in the disease. It is of great significance for CRP to the clinicaldiagnosis of the coronary heart disease. |
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