| Ilex rotunda thunb, which is also known as rotunda thunb is an aquifoliaceae plant, also known as iron holly, in southern China is the common Chinese herbal medicine, the bark or leaves are used as medicine, has the clearing heat and cooling blood, anti-inflammatory, analgesic effect, early mainly as commonly used traditional Chinese medicine in the treatment of various infectious diseases and gastrointestinal diseases. Because of the acute disease curative effect and exact, recognized as the first aid medicine. In recent years, the relevant literature, Ilex rotunda thunb the extract has effect on the treatment of hypertension, coronary heart disease, but research on the effective part is very rare. Ilex rotunda thunb contains many medicinal ingredients, including pedunculoside is a representative compound three saponins from it, but now the research on pedunculoside quality standard is still not perfect, there is no uniform standard to follow in the production of reliable, so we set up the quality standard of pedunculoside, at the same time in order to control the quality of raw material, this experiment established jiubiying fingerprint medicinal materials.This experiment using high performance liquid chromatography method was established for determination of content of pedunculoside method, through the study on the method validation instrument good precision, reproducibility and recovery rate are in line with the requirements of method, in addition according to the relevant item set up quality standard of Pharmacopoeia of the habitat, with winter anthocyanin were traits examined, TLC identification, related substances and magazines to check. Traits examined in pedunculoside API color, flavor, toxicity of state description. The TLC is sample preparation for raw material medicine according to pharmacopoeia, the chromogenic agent relevant and appropriate temperature to make the qualitative identification of the related substances, check whether the main influence of raw material purity of the samples meet the requirements, the experimental results in order to prove that the application of the material sample are reliable, convincing. Journal check in Pharmacopoeia of raw materials for loss on drying, residue on ignition, heavy metals, arsenic salt internal indicators such as inspection, the allowed range of provisional indexes, thus the tentative quality standards of pedunculoside.The experiment also screened out from different habitats of the 10 batch of holly bark, established by the high efficiency liquid chromatography fingerprint method. First, through the consult massive related literature, the holly bark with different extraction methods, different solvent and different extraction time were investigated, through the HPLC method for the determination of various conditions it should save the separation effect of effective components, select the best extraction method; then in the selection of chromatographic conditions, the column temperature, column setting the detection wavelength, different conditions such as different types or different manufacturers were investigated respectively, and ultimately determine the optimal chromatographic conditions. After get the best chromatographic condition experiment:chromatographic column was Apollo C8 (4.6mm×250mm,5μm); using acetonitrile and water as the mobile phase with gradient elution; column temperature:30℃; the detection wavelength:210nm; sample 5μL; the flow rate was 1.OmlVminAfter screening conditions of extraction and chromatographic conditions, on 10 different areas of jiubiying medicinal materials fingerprint study. Study on the method of the peak of the relative retention time and relative peak area were in conformity with the provisions, the method can be used for the study of fingerprint. Establishment of jiubiying fingerprint medicinal materials,9 common peaks, the chromatographic fingerprint similarity evaluation software version 2004 A similarity calculation, the 10 batch of holly bark similarity is greater than 0.9. The medicine of good similarity. The number of chromatogram peak is basically the same, but the peak area of chromatogram of different, the ten batch of medicinal components of roughly the same, but the drug content has very big difference, this explains the different areas, different years as light moisture content difference, medicine will be very different.Through the establishment of jiubiying fingerprint herbs can effectively control the holly bark quality, selection of high-quality trueborn medicinal materials as raw material drug research, provide scientific basis for drug R & D related follow-up. |
PDF Full Text Request