Studies On The Pharmacological Effect Of Processed Sibiraea On Immunologic Function And Anti-fatigue

Sibiraea is a commonly used drugs for the Tibetan people, which belongs to the Sibiraea angustata (Rehd.) Hand-Mazz.and Sibiraea laecigata (L) Maxim.of Sibiraea of Rosaceae. Foliage and fruit often was used as (with fruit branch) medicine for Tibetan.Mainly used for digestion, dispelling wind and heat; Tibetan medical literature recorded:Sibiraea can treat fever and disease,which grows at an altitude of more than 3000～4000m in the bushes or sand and gravel, and distribute in southern Qinghai,southern and western Gansu, western Sichuan and northwestern Yunnan.We process Sibiraea by traditional method.One of the methods was utilized in Tibetan nationality folk which was simmering over low heat.But the modern pharmacological research of Sibiraea is indefinite.Recently,more and more people focus on the immune regulation of plant polysaccharides.And there are hundreds of herbs used for the correlative research. At the same time, the research on anti-altitude sickness of Tibetan drugs is also little. Otherwise, the development of herbs exerts severe pressure on the natural populations.. So we use leaves and fruit of Sibiraea for our research to ensure the sustainable utilazation of Tibetan durgs resources.Our laboratory has a long-term research on the production of Gansu Rosaceae. Later we stydy the immune and anti-fatigued function of the processed Sibiraeas polysaccharide. Preliminary results indicate that processed Sibiraeas polysaccharide can increase the immunity, and the water extract can help the body to resist fatigue.Our group extract water-soluble polysaccharide from processed Sibiraea, and observe its effect on Cyclophosphamide immunosuppression model of mice. After the treatment,The organ weight index and the delayed type hypersensitivity of the mice were investigated.As well as,humoral immune function was determined by serum hemolysin assay.Non-specific immune function was determined by carbon clearance method.And Cellular immune function was determined by Spleen lymphocyte proliferation test.Effect of Polysaccharides in Processed Sibiraea on immunologic function of immunosuppression mice, Two hundred kunming mice(20±2g) which male is 50 percent were randomly divided into five groups:normal controls,model group,low-dose group [110mg/(kg.d)],middle dose group [220mg/(kg.d)],high-dose group [440mg/(kg.d)],drugs were given to the mice by oral gavage every day. The immunosuppressed mice treated with Sibiraea polysaccharide at intragastrica dose of 110-440mg/(kg.d)can increased the weight of the immune organs,the content of DTH and the content in serum hemolysin lgG lgM.As well as,the rate of carbon clearance was enhanced and the proliferation of spleen lymphocyte was increased.The research group made a pharmacological anti-fatigue experiment, to provide experimental basis for the further development in theory. In the experiment, the research group made mice swimming with heavy loads which is anima model.After the research group determine the liver/muscle glycogen of content, observe the mice swimming time for macro-endurance.The pharmacological anti-fatigue experiment:A hundred Kunming male mice(20±2g),which were randomly divided into five groups:normal controls, low-dose group (0.95g/kg.d), middle dose group (1.9g/kg.d), high-dose group (3.8g/kg.d). The results showed that the Sibiraea group whoes endurance and liver/muscle glycogen of content increase Significantly. The research group made conclusion that the Sibiraea group can improve macro exercise tolerance and increase liver/muscle glycogen reserves.