| For the study of drought resistance difference in germplasm Brassica campestris from different ecological environment in Qinghai, identificate drought-resistant germplasm and screen related genes in strong drought resistance Brassica germplasm. The Brassica napus germplasms was collected in different ecological environment of 35 parts, the main results of the drought resistance identification, isolation, identification and expression of drought resistance fragment analysis are as follows:1.Drought Resistance Trial Germination of 35 indicators will be based on each test parts Brassica napus germplasm sort found after drought resistance, there are some differences in drought resistance of rapeseed resources under different ecological environment. Hydroponic experiments and drought seedling pot experiment ordering4 germplasm for further PEG Stress validation experiments shows that the germination identification of drought was real and effective, the top 4 varieties of oilseed rape germination stage and seedling drought resistance was stronger than other resources;2.The separation and identification of drought- related fragments shows that the drought-related gene expression determines the plant’s drought resistance. Selected from the strongest drought resistance germplasm candle Rapeseed drought stress increases the fragment 27, down 17 fragments.In 27 fragments, 21 fragment was cloned successfully, in 21 fragments, 20 fragments were highly similar with brassicas.3.The expression analysis use β-actin as a reference gene, primers were designed from screening of X2-7 fragments, using real-time quantitative RT-PCR experiments on 31 material, Brassica rapa F-box protein SKIP16-like gene in leaf, cotyledon, root and normal supply conditions in a 20% concentration of PEG Stress leaf, cotyledon, root expression patterns were analyzed. The results shows that under normal growth conditions, X2-7 gene express in a certain amount of PEG stress, in drought stress induced upregulation of expression and the higher amount expressed in the cotyledon. These results suggest that the expression of X2-7 genes induced by drought, is related genes in Brassica campestris of drought and saving water resources. |
