Analysis Of Differential Expression Genes By CDNA-AFLP Approach During Drought Stress In Sunflower Seed Germination

Drought is one of the important factors limiting plant growth and yield improvement.Sunflower,as an important economic value of the crop,its drought has been the concern of domestic and foreign researchers.In this study,18 sunflower materials were selected as test materials.The germination test and germination and germination index were used to determine the sunflower materials with the strongest drought tolerance.The differentially expressed genes of sunflower seeds under different drought stress were screened by cDNA-AFLP technique for strong drought-resistant sunflower materials,and the possible reasons for drought resistance were revealed.The main conclusions of this study are as follows:(1)18%PEG-6000 simulated drought stress,the relative germination potential,relative germination rate,relative germination index,relative radicle length,relative germ length,relative germination weight,Relative MDA content and relative ATP content were significantly correlated with the comprehensive evaluation value of membership function,indicating that these indicators could be used as the main reference index for drought resistance screening of sunflower germination period.(2)The drought resistance of strain 18 was 1.49,the drought resistance of strain 184 was the weakest,D was 0.36,and the recombinant inbred lines were 88,35.29,64 D value greater than the parents K55 and K58,more than non-parent K59,indicating that the strain 88 can be used as a drought-resistant materials for the next test.(3)A total of 1218 polymorphic bands were amplified by 35 pairs of primers with good polymorphism.About 35 bands were clearly visible on each pair of primer combinations,and the bands were mainly concentrated in the range of 100?1000 bp The Among them,a total of 300 TDFs were obtained,accounting for 25%of the total polymorphic bands,and the fragment size was mainly distributed between 100 and 750 bp.(4)The 300 uptrended TDFs were re-amplified to 116 clear and single fragments,accounting for about 39%of all secondary amplification products.Twenty-one positive TDFs were obtained from 116 TDFs amplified by reverse PCR by reverse blotting,and four TDFs were 3 days of stress,and 9 for TDDs at 5 days of stress,indicating that the 13 positive TDFs could be Drought Stress Induced Specific Expression Genes.(5)Of the 13 positive TDFs,10 TDFs were homologous to known sequences,and were mainly involved in transcriptional regulators,ribosomal proteins and signal transduction,and the other three TDFs were not found in the database for homologous protein sequences,Which may be drought-related genes for unknown sequences.