Analysis Of Alkaloid Component And Toxicity Test In The Aerial Parts Of Ligularia Rumicifolia

For a long time, affected by overgrazing of natural grassland and selective grazing of livestock, the plants of genera Ligularia Cass. have been flourished in alpine meadow. In virtue of allelopathy, the genus not only inhibiting the growth of high quality forage, but also other toxic and inedible pasture grass, and carried the changing of grass community structure along with them.Thus the genus became the dominant population in the prairie, which has become one of the hallmarks of grassland degradation. Sprindling of Ligularia rumicifolia has been severely eroded the growth high-quality forage, resulting in the deterioration of grassland production and intensifying feed-animal imbalance, which poses a serious threat to the sound development of animal husbandry and ecological balance of grassland. By now, the study of its chemical components and toxicity haven’t been reported. Ligularia rumicifolia was studied in this experiment, measured alkaloids of Ligularia rumicifolia by GC-MS. By toxicity test in mice, LD50 was determined, serum physiological parameter and histopathology were observed, evaluate toxicity of Ligularia rumicifolia in mice, it was provided theoretical evidence for toxicology, mechanism of toxicity, utilization prospect of Ligularia rumicifolia. In this study, following results were obtained.1. Analysis on alkaloids: isolated and measured alkaloids of Ligularia rumicifolia by alkaloid extraction, TLC, column chromatography and GC-MS. The results were as follows: The rate in the parts of acidity chloroform, alkaline chloroform and alkaline n-butyl alcohol was 0.55%,0.31% and 16.58%, respectively. By thin layer chromatography analysis, there were 2 alkaloids in acidity chloroform, 4 alkaloids in alkaline chloroform,1 alkaloid in alkaline n-butyl alcohol. By column chromatography and GC-MS, there were 4 alkaloids, senecionine, integerrimine, platyphylline and erucamide, in chloroform.2. Acute toxicity test in mice: conducted to evaluate the acute toxicity of Ligularia rumicifolia by intragastric and intraperitioneal injection in mice. The results were as follows: the LD50 of gavage and intraperitioneal injection were 91.69 g/kg and 15.75 g/kg respectively, its confidence limit was 83.10 to 101.16 g/kg and 14.01 to 17.72 g/kg respectively. Combined with clinical symptoms of poisoning and the pathological changes in mice, a comprehensive analysis indicates that Ligularia rumicifolia has toxic effect, the primary target organ were gastrointestinal tract, liver and spleen.3. Subchronic toxicity test in mice: fed mice by different proportions of aerial parts of Ligularia rumicifolia, Clinical manifestation, feed intake, weight, organ coefficient, serum physiological parameter and histopathology were observed. The results were as follows: 10% Ligularia rumicifolia add in feed, there were no obvious toxic injury in heart, liver, spleen, lung, kidney, cerebral, cerebellum, testis and ovary of mice. Adding 2.5% or 10% Ligularia rumicifolia can lead to putting up weight of mice, during the first 12 weeks.