Alkaloids Constitutents Research And Toxicity Evaluation Of Oxytropis Falcate Bunge

Oxytropis falcate Bunge also named Da Xia in Tibetan medical herd which is perennial herb that belongs to Oxytropis of Leguminosae. It has broad prospects for development of medicinal . The chemical composition of Oxytropis falcate Bunge have been reports more, but its toxicity study haven't been reported. This research applied the chemical constituents of systemic pretesting and TLC techniques to identify types of chemical constituents from Oxytropis falcate Bunge. Through mutagenesis experiments, sub-chronic toxicity test and three of the reproductive test to research and evaluation the toxicity to prove that the Oxytropis falcate Bunge like locoweed providing strong support, as well as the development of drugs to provide more in-depth information. The experimental results were given as follows.1. Pre-experiment on Chemical Composition and TLC alkaloids analysis of Oxytropis falcate BungeThe chemical constitutes was preliminary tested though systematic and special pre-experiment. The results show that plenty of Phenols, tannins, alkaloids, flavones and its glycosides, saccharides polysaccharides and glycosides, amino acids and opypeptides, saponins, organic acids, sterides and triterpenes, volatile oils exist in Oxytropis falcate Bunge, does not contain anthraquinone and its glycoside, lactone, coumarin and its glycoside, cardiotonic glycoside, cyanophoric glycoside and aliphatic series nitrocompound.Through systematic extraction of alkaloids and selection eligible development system, TLC was used to analyze alkaloids that extracted by different solvent. The results indicated that the major alkaloids in Oxytropis falcate Bunge is indolizidine compounds, furthermore, there are minimum, neutral and maximum polarity indolizidine alkaloids and the maximum polarity alkaloids hold the first place. The extractum extracted by chloroform contains 6 species alkaloids, The fraction of EtOAc contains 11 species alkaloids, The extractum extracted by n-butanol contain 5 species alkaloids. In three extracted fractions of Oxytropis falcate Bunge all contain swainsonine compared with standard preparation, and obtain 28 mg Swainsonine from n-Butanol Extractive, extraction rate 0.00187%. 2. The Mutagenicity Test of n-Butanol Extractive of Oxytropis falcate Bunge in miceThe mutagenicity of three gradient aqueous solution 800 mg/kg, 1 000 mg/kg, 2 000 mg/kg, 4 000 mg/kg of n-Butanol extractive from Oxytropis falcate Bunge were evaluated by PCE micronucleus test and sperm abnormality test. The result showed that n-butanol extractant from Oxytropois falcate Bunge induced the micronucleus rate were 2.625‰, 4.625‰, 6.50‰and 12.75‰and sperm abnormality rate were 1.44%, 1.99%, 3.40% and 3.475%, moreover have a dose-response raletionship. It means that low-concentration extractant from Oxytropis falecate Bunge, which safety range was below 2 000 mg/kg, have not mutagenic effect and genetic toxity to micronucleus cells and sperms in mice, but high-concertration have mutagenic effect .3.Subacute Toxicity Test of Oxytropis falcate Bunge in MiceThis study fed mice on feedstuff which added Oxytropis falcate Bunge powder, passed through period of time to mice clinical symptom observation, the blood convention coefficient examination, detectioning peripheral blood T-lymphocyte and peritoneal macrophage swallow function, autopsy, studies the effect of Oxytropis falcate Bunge to mice. Result showed that white cell number and the hemoglobin have the certain influence, but the lymphocyte and granular cell's influence were not different. The peripheral blood T-lymphocyte ANAE positive stain of trial groups were 8.4%, 15% and 18.5%, compared to control group has remarkable difference(P<0.05). This study observed that the peritoneal macrophage swallow function was enhanced with low Oxytropis falcate powder content foodstuff, but it was inhibit with high content, phagoindex of macrophagus were 0.86, 0.47 and 0.32. Furthermore, cytoplasmic vacuolation was observed in parenchymal cell of varoius organs that sample from Oxytropis falcate Bunge feeding mouse.4.Oxytropis falcate Bunge reproductive toxicity testOxytropis falcate Bunge feeding mice three reproductive toxicity test results showed that the Oxytropis falcate Bunge pregnancy weight gain of animals have a marked impact, the higher the plant contented the pregnancy mouse body weight gain more significant difference (P<0.05), from embryos, bone and offal deformity greater probability that the Oxytropis falcate Bunge with other Oxytropis grass, with organ damage, or even reduce male's sexual activity, to the dams abortion, fetal weak production, teratogenic, and other reproductive toxicity.