Microbial Diversity Of Soil Infected By Meloidogyne Spp

The Meloidogyne has a great influence on the productivity of the crops. In this study, constructing rRNA gene clone libraries and Illumina MiSeq sequencing were used to investigate the diversity of bacterial and fungal communities in soils infected by the Meloidogyne. The purpose of this study was to research the relation between microbial community structure with Meloidogyne invading. The results were as follows:1.We have analyzed the bacteria community structure in Meloidogyne soils. The four most populated bacterial phyla are Proteobacteria, Crenarchaeota, Actinobacteria and Chloroflexi. The dominate bacterial familiae are Nitrososphaeraceae. Gaiellaceae, Xanthomonadaceae, Nocardioidaceae, Gemmataceae and Pseudomonadaceae. The dominate bacterial genera are Candidatus Nitrososphaera, Pseudomonas, Acinetobacter and Rubrobacter. Compared with other yuanmou soils, the percentage of Proteobacteria, Actinobacteria increase, but one of Crenarchaeota, Nitrososphaeraceae, Candidatus Nitrososphaera, Xanthomonadaceae and Pseudomonadaceae decrease in the soisl infected by Meloidogyne seriously. The invasion of Meloidogyne narrowed the diversity of bacteria. The percentage of Proteobacteria, Actinobacteria, Pseudomonadaceae, Nitrososphaeraceae, Candidatus Nitrososphaera and Pseudomonas has a remarkable change with Meloidogyne invading.2. We have analyzed the diversity of bacteria in different time soils with Meloidogyne invading. The four most populated bacterial phyla are Proteobacteria, Crenarchaeota, Actinobacteria, Chloroflexi. The dominate bacterial familiae are Nitrososphaeraceae, Xanthomonadaceae, Micrococcaceae, Comamonadaceae, Chitinophagaceae, Nocardioidaceae. The dominate bacterial genera are Candidatus Nitrososphaera, Kaistobacter, Rhodoplanes, Hydrogenophaga, Nitrospira. The diversity of bacteria is more abundant as time goes on. By Meloidogyne invading, the relative abundance of Crenarchaeota, Nitrososphaeraceae, Candidatus Nitrososphaera decrease, but one of Chloroflexi increase. It has been found that the bacterial richness and diversity in soils infected by Meloidogyne are obviously lower than that in the healthy soils. 3.We have analyzed the diversity of fungi in soils with Meloidogyne invading. The three most populated fungal phyla are Ascomycota, Zygomycota. Basidiomycota. The dominate fungal familiae are Mucoraceae, Pleosporaceae, Incertae_sedis, Mycosphaerellaceae. The dominate fungal genera are Alternaria, Myrothecium, Cladosporium, Rhizopus, Volvopluteus. The relative abundance of Ascomycota, Pleosporaceae, Incertae_sedis, Alternaria and Myrothecium increase. It has been found that the fungal richness and diversity in soils infected by Meloidogyne are obviously higher than that in the healthy soils. The percentage of Rhizopus, Aspergillus, Cladosporium and Volvopluteus is relative big in soils by Meloidogyne invading slightly.4.Many bacteria, actinomycetes and fungi were separated from nematodiasis soils based on traditional microbial culture. By the bioassay, we have identified microorganisms whose pathopoiesis against Meloidogyne are relative higher. We have found there are similar cultured microorganisms in different yuanmou soils.We can draw the conclusion that Meloidogyne disease can greatly impact on the diversity of microbes. The results not only provide a theoretical and technical support for future studies of Meloidogyne soil, but also enable us to have a more comprehensive understanding of the microbial community structure of Meloidogyne soil to provide important resource for the study of Meloidogyne disease biocontrol. Highlight of this research:1.We have analyzed the difference of rhizosphere bacteria in different time soils by means of metagenomics.2.We have reported that rhizosphere mycobiota have much difference between healthy soils and nematodiasis soils by means of metagenomics.