| The objective of the study is to investigate the temporal dynamic change of soil physiochemical characteristics and microbial community composition and structure,as well as relationship between microbial community structure and diversity in rhizosphere of strawberry and soil environmental factors.The aim of this study was to explore the temporal dynamic changes of soil physiochemical characteristics and microbial community composition and structure by means of measuring soil physiochemical characteristics and microbial community composition and structure with strawberry rhizosphere soil of different continuous cropping years in various growth period,to provide theoretical basis for the control of strawberry continuous cropping obstacles.The main results were as follows:1.Temporal dynamic changes of soil physiochemical characteristics of different continuous cropping years were not significant.Temporal dynamic changes of organic matter,Alkali-hydrolyzable nitrogen and available phosphorus were not significant,available potassium showed a descend trend during fruiting and harvest stage,pH was at the least level during fruiting stage and harvest stage,less than other stages.Soil nutrient content had differences among different continuous cropping years,showed that soil nutrient content of continuous cropping years were higher than contrast.2.Diversity and abundance of bacterial and fungi showed differences among different continuous cropping years and growth stages.Diversity and abundance of bacterial of soil without continuous cropping(CK)were the highest during harvest and end growth stage as well as lower during bud and harvest stage.Diversity and abundance of bacterial of soil with continuous cropping for 1 year(1Y)were the highest during the end growth stage,diversity and abundance of bacterial of soil with continuous cropping for 8 years(8Y)were higher during seedling stage,lower during harvest stage.Diversity and abundance of bacterial of 1Y were higher than CK and 8Y.Diversity and abundance of fungi of CK were higher during end growth stage,lower during seedling stage.Diversity and abundance of fungi of 1Y were the highest during seedling stage,least during fruiting stage.Diversity and abundance of fungi of 8Y were the highest during seedling stage,lower during harvest stage.3.Bacterial community composition and structure showed differences among different continuous cropping years and growth stages.During the whole growth period,dominant bacterial of CK were Actinobacteria,Proteobacteria,Firmicutes,Cyanobacteria,Bacteroidetes,Chloroflexi and Acidobacteria.Dominant bacterial of 1Y were Actinobacteria,Proteobacteria,Firmicutes,Cyanobacteria,Bacteroidetes,Chloroflexi,Acidobacteria,Planctomycetes,Gemmatim-onadetes and Verrucomicrobia.Dominant bacterial of 8Y were Actinobacteria,Proteobacteria,Firmicutes,Cyanobacteria,Bacteroidetes,Chloroflexi,Acidobacteria,Planctomycetes and Gemmatim-onadetes.Dominant rhizosphere bacteria of different continuous cropping years trend to be single.4.Fungal community composition and structure showed differences among different continuous cropping years and growth stages.During the whole growth period,dominant fungi of CK were Ascomycota,Basidiomycota and Chytridiomycota.Dominant fungi of 1Y were Ascomycota,Basidiomycota,Chytridiomycota and Zygomycota.Dominant fungi of 8Y were Ascomycota,Basidiomycota and Zygomycota.Dominant rhizosphere fungi of different continuous cropping years trend to be single and had a big change during the whole growth period.5.Soil physiochemical characteristics were closely related with different dominant microbial community.In this research,the order of effects of soil physiochemical characteristics on bacterial community composition and structure was organic matter>available potassium>pH>available phosphorus>Alkali-hydrolyzable nitrogen.The order of effects of soil physiochemical characteristics on fungal community composition and structure was available potassium>organic matter>Alkali-hydrolyzable nitrogen>pH>available phosphorus.Proteobacteria had a significant negative correlation with pH(P<0.05);Acidobacteria and Planctomycetes had a significant positive correlation with organic matter(P<0.05);Chloroflexi,Acidobacteria and Planctomycetes had a significant positive correlation with Alkali-hydrolyzable nitrogen(P<0.05);Verrucomicrobia had a significant negative correlation with available phosphorus(P<0.05);Chloroflexi had a significant positive correlation with available potassium(P<0.05);Ascomycota had a significant negative correlation with organic matter and Alkali-hydrolyzable nitrogen(P<0.05);Zygomycota had a significant positive correlation with available potassium(P<0.05). |
