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Study On Soil Microorganisms In Oiltea Camellia Rhizosphere

Posted on:2014-05-23Degree:MasterType:Thesis
Country:ChinaCandidate:M SangFull Text:PDF
GTID:2323330488464776Subject:Forestry
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Though research to the 15 species of soil rhizosphere microorganism in Tongkou Forest Farm. Discussed the relationship with microbe quantity, species and soil active. The main results were as follows.1. Adopt the method that the soil suspended a liquid to carry on separation with the different flat panel method, the amount of soil microorganism were respectively:bacteria> actinomycetes>fungi. Camellia caudata Wall had the highest number, Camellia polyodonta lowest number, every grams of dry soil in the number of 0?1.71×103cfu·g-1, the average number was 0.86×103cfu·g-1. Actinomycetes mean differences the most significant, Camellia caudata Wall samples of actinomycetes content was highest,14.2×103cfu·g-1, the lowest Camellia polyodonta samples,0.1×103cfu·g-1, the average number of colonies for 7.1×103cfu·g-1. Camellia nanyongensis Hu samples per gram dry soil bacteria found in 1.81×103cfu-g·1. Camellia polyodonta was the soil samples of the 0.73×103cfu-g·1, the average number of bacteria was 1.27×103cfu·g-1. Ammonifier maximum levels for large Camellia furfuracea was 6.9×103cfu·g-1, Camellia uraku sample of monomer content of 0.9×103 cfu·g-1, the average number was 3.9×103cfu·g-1. Phosphate-solubilizing bacteria for Camellia meocarpa were 4.4cfu·g-1, the lowest for 1.Ocfu·g-1 was Camellia nanyongensis, the averagenumber was 2.7×103cfu·g-1.Nitrifying bacteria could Camellia nanyongensis content was highest,7.2×10 cfu·g-1, Camellia oleifera Abel was the lowest number 1.0×103cfu·g-1,the average number was 4.1×103cfu·g-1.the large number of Potassium bacteria was Camellia taishunensis,4.85×103cfu·g-1, the least amount of Camellia polyodonta 1.32×103cfu·g-1,the average number was 3.09×103cfu·g-1. Cellulose decomposing microorganism by the number of Camellia nanyongensis was 2.87×103cfu·g-1 with the least number of Camellia meocarpa were 0.04×103 cfu·g-1, the average number of cellulose decomposing bacteria in soil was 1.46cfu·g-1. Nitrogen fixing bacteria content in the highest sample for Camellia caudata 0.29×103cfu·g-1, the lowest for the Camellia meocarpa, Camellia oleifera, Camellia crapnelliana is zero, the average number was 0.15×103cfu·g-1. Comprehensive analysis of Camellia uraku, Camellia furfuracea, Camellia taishunensis, this was a few samples in the most abundant microorganism quantity.2. This paper stated how to isolate and purified the organism and soil microorganism separation, identify fungal cultures including 18 genera and 11 families,10 orders and 4 classes.Actinomycetes mainly was divided into seven main physiological groups, producing actinomycetes actinomycetes groups, purple color groups, water absorption actinomycetes groups, funicola actinomycetes groups, cyan actinomycetes groups, whorled actinomycetes groups, yellow actinomycetes groups.3. To study the activity of soil respiration in the largest release a quantity to 1.40 mg, minimum to release a quantity to 0.68 mg, respiration to release a quantity to a mean of 1.00 mg; Buried slice method was used to determine the strength of cellulose decomposition, decomposition intensity was 4.87%, the highest decomposition intensity was 0.58%, the lowest average decomposition intensity was 1.70%. Using soil total nitrogen determination method to determination of the conversion of nitrogen in the soil, the highest conversion rate was 40.87%, the lowest conversion intensity was 5.80%, the average transformation intensity was 20.03%, the effective phosphorus efficiency, the highest intensity of phosphate-solubilizing efficiency was 12.33%, the lowest value was 2.27%, the average intensity of phosphate-solubilizing rate was 7.09%.If the decomposition and the transformation ability was best,it just explained the variety of soil microbes had largest number and the decomposition transformation effect was strongest.
Keywords/Search Tags:camellia, rhizosphere, soil microorganism, fungi, actinomycetes, bacteria
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