Optimization Of Culture Medium And Reduce Blood-lipid Of Armillaria Mellea

Armillaria (scientific name: Armillaria mellea) is part of the Fungi, Basidiomycota, and it is the ssential symbiotic bacteria of a valuable medicinal plants Tianma and grifola’s growth process. In recent years,the medicinal value and fluorescence of Armillaria attracted wide attention. In medical field,it can calm center, improve sleep, enhance immunity and regulate nerve and related products is an effective drug treatment of headache, dizziness, weakness and other diseases. Luminescence is one of the important features of Armillaria, in sufficient oxygen, proper humidity, temperature and pH, the young tender parts of vigorous growth Armillaria will send white and green fluorescence, the light is a cold light.No thermal, for bacteria in chemical energy directly into luminous energy. Armillaria luminescence properties have been used as genetic markers and biosensors, foreign reports the luminescence properties can be used to detect the environment, but the research on Armillaria bioluminescence optimal living conditions is very few.So to find the optimal culture conditions conducive to the growth and light of Armillaria, fast development of high yield and high quality Armillaria bacterial strain will play an important role to promote the development of fungus Armillaria industry. This study is conducted to explore the culture conditions in carbon source, temperature, PH value, the effect of light on the growth of Armillaria and luminescence properties to do some basic work for the realization of quickly cultivate Armillaria.1.Respectively,substitute the basal medium fructose with glucose,starch,galactose,lactose,sucrose of different carbon source to prepare five groups of fermentation medium.We found that in the starch medium, rhizomorph germination time is the shortest,the growth is the fastest,and the rhizomorph is developed,the highest content of lipopolysaccharide.The test proved to starch as the carbon source is the best choice.2.Prepare culture medium5ml, adding to35mm diameter Petri dish, inoculated, respectively, in5℃,15℃,25℃,30℃fermented, measure luminous and the growth rat e of mycelium, the temperature have a significant impact on the luminescent and the growth of Armillaria,the most suitable temperature for the growth and luminousof Armillaria is25℃.3.Prepare culture medium5ml, adding to35mm diameter Petri dish, inoculated, cultured in different photoperiod,24h light,12h light/12h dark,24h dark, light affect the luminous of mycelium,but does not affect the growth of Armillaria.The highest luminous brightness of armillaria culture is without illumination conditions, followed by illumination12h lighting culture conditions, the dimmest luminous brightnessare completely lighting culture.4.Prepare culture medium5ml, pH adjusted to3.0,3.5,4.0,4.5,5.0,5.5,6.0,7.0after sterilization, was added to a35mm diameter Petri dish, inoculated, culture in a dark place at a temperature of25℃, when the PH value is3.5, it’s the most suitable conditions for Armillaria luminous, when PH value is5.5.mycelia have the weakest lightness, when the PH value is4.5,the growth rate of the mycelium is the fastest.5.Armillaria polysaccharide extracted150mg/kg,100mg/kg continuous feeding rat model of hyperlipidemia, it can significantly reduce the serum of high fat rats, levels of TG,TC and LDL-C (P <0.05). Armillaria polysaccharide also increases the serum HDL-C levels of fat in rats. The experimental results confirm Armillaria Table polysaccharide have certain lipid-lowering effect.