Tianma, The Armillaria Allelopathic Phenomenon Tianma Even As Barriers To The Reasons Discussed

There is a serious continuous cropping obstacle in the production of the famous Chinese herb Gastrodia elata BL. In order to find out the reason, the research is about the allelopathy in the Gastrodia elata BL field soil. Taking rapeseed as the receptor by means of biological measurement we studied the allelopathy of soil water extract, Gastrodia elata tuber water extract, Armillaria mellea metabolites, We studied the effect of Armillaria melle culture on the growth of itself. And we also studied the change of soil microorganism by year.The results are as follow:The soil water extract has an effect of inhibiting germination on the rapeseeds. When the level is too high or too low, it will reduce the length of epicotyels and hypocotyels. The soil water extract can increase the total biomass in 50% concentration, but reduce at a too high or too low level.Gastrodia elata tuber water extract has an effect of inhibiting germination on the rapeseeds. It can increase the length of seedling. The influence on seedling, hypcotyels, and total biomass presents to be low-promoting and high-repressing.The results of liquid culture showed that when the nutrient is enough the Armillaria mellea will keep on growing. And new metabolites occur in the culture. Armillaria mellea metabolites (Including pelleted mycelia metabolites, rhizomorph metabolites, metabolites from different periods) have an effect of inhibiting germination on the rapeseeds. The effect on the seedling, hypcotyels, and total biomass are different: Pelleted mycelia metabolites can increase the length of epicotyels, and can reduce the length of hypcotyels. The effect on total biomass is low-promoting and high-repressing. Rhizomorph metabolites can increase the length of seedling and hypcotyels, and it can also increase the total biomass. Armillaria mellea metabolites from different periods all have an promoting effect on the growth of epicotyels and total biomass. Only the 17d's culture inhibits the growth of hypcotyels while others promote.Armillaria mellea liquid fermentation can promote the growth of pelleted mycelia. And the influence on aging is low-repressing and high-promoting in liquid culture. While in solid culture the Armillaria mellea liquid fermentation will repress the growth of rhizomorph. High level Armillaria mellea liquid fermentation in the solid culture can promote aerial hyphae and has an anti-aging effect on the rhizomorph.The number of soil microorganism in high altitude area in Gastrodia elata BL field is small. The species and quality are different in the soil being removed for different years.