The Research Of The Stem Tip Virus-frree Culture On Potato

In this experiment, use the potato apical meristem as the experimental materials, a single reagent and combination of reagents for disinfection of potato shoot tip pretreatment, disinfection reagents include alcohol, bleaching powder, mercuric chloride, sodium hypochlorite, calcium hypochlorite and peroxide of hydrogen six kinds of reagents. By analyzing the growth of the plant contamination and survival to determine the effect of disinfection. Further optimization of the number of long and sterile water disinfection, take ethanol for30s,60s and120s, sterile water wash2,4and6times, mercuric chloride treatment2min,3.5min and5min constitute the three factors and three levels is cross experiment results show that the chloride Hg3.5min, alcohol treatment120s, sterile water six times, potato virus-free seedlings sterile survival rate of up to90%, based on contamination and survival to determine the optimal sterilization portfolio or more.Using the yellow "potato NO.l", white potato,"early and Large White", Purple potato "purple sweet potato", survival after the apical meristem by the detoxification of different varieties of culture, the results showed that the issue of the survival rate can reach70%,0%survival rate in the purple sweet potato, there are significant differences show that different species of stem apex detoxification effectiveness and efficiency.With different types of explants shoot tips the detoxification culture on "potato NO.1", including the mother potato, shoot tip three kinds of lateral buds of the lateral buds on the plants of the natural environment and incubator detoxification seedlings. The results show that the mother potato shoot tip detoxification cultivate a seedling high contamination rate, the growth of a relatively poor survival rate of only50%; incubator after detoxification pretreatment lateral bud growth in the sterile environment compared to the natural environment of seedlings growing strong, but the survival rate of80%, so no vaccine lateral bud tip detoxification cultivate the best.Optimization of media components designed in MS medium with different concentrations of hormone PIX and juice additives test results show that the hormone PIX to promote virus-free potato seedlings to shorten the section, increased stem diameter, and inhibition of leggy to promote strong seedlings have a significant effect. PIX concentration of0.5mg/1and1.0mg/1, potato detoxification seedlings grow strong, branching up to the leaves up, and the color is green, some is dark green; the juice of different concentrations of additives on the detoxification seedlings have the duty to promote or inhibition, it provides some essential trace nutrients and physiologically active substances and growth substances, the experimental low concentrations of radish juice helps the growth of virus-free potato seedlings.