Technological Study On High Efficiency Of Virus-free Recovery Of Potato By Meristem Tip Culture, Rapid-micropragation And Microtubers Multiplying

The study was done for the need of high efficiency of viruss-free recovery of potato by merism culture, rapid-micropragation and microtubers multiplying. The mortified method for meristerm culture ,the selection of medium in rapid-propagation of plantlets,the way of microtubers inducing .dormancy breaking of potato were studiedIn the study of disease-free recovery of potato meristem culture .The effect of tempture, size of meristem hormones,activated charcoal in meristem culture were studied the result shows that treating sprout tubers with 38 ℃ for 5 weeks, exercising the mirestem with 2 primodial, putting it into the madium of MS+BA2mg. L-4NAA0.1mg. L-4GA3 0.lmg . L-1+0.05%AC+3%suger+6%Agar can meet the need of factory production. It spent 130 days far plant forming/The ratio of callors and sprouting was about 65%averagy 5-7 adventitions sprout grew from every meristerm.Three genotype in virto plants were rapidly propagated by 20 kinds of mediums which were different from each other in concentration of salt,organic substance hormones,culture medium sustainer.lhe change of growth was investigated and analysisedThe result show that the growth speed of three genotype were different ,but they were responded similar to different mediums and in virto plants grew normally in liquid medium of MS macro-ements+3% edible-suger+Fe-salt +0.2 mg L-1IBA+l0 mg ?L/'B, sustained by cotton .Growing state of root, stem, leaf were similar to high-cost MS medium with complex ingradiencesJt can be used for rapid-propagation of in vitro plant in mass production.The study of potato microtubers inducing included two aspects.In the study of in vitro tuber inducing.'varies treaters related four factors of temperature, light, horrnones,activated charcoal were disposed Investigation were done in the period of growth and harves The result indicated liquid media sustained with cotton of MS+8%suger+5-10 mg . L'BA-K).1% mg ?I/'AC or MS+8% edible-suger -+60ppm vallin+0.1%A.C, temperature of 22℃ a11 light in first 5days then in dark with 18℃ were the best culture conditions for tuberization of in vitro tubers. In the research of minhubers inducing by cuttings without soil, composition of rooting liquid and nutrition liquid of minitubers inducing were studied The result indicated NAA50 mg-L'M-IBA50 mg-L'+GA3 3mgL-1 used as rooting liquid could achieve high living ratios. 20gNO(NH2)j+40gCakium superphosphate -40gKCl was used for macro-eternents irrigating once a week and (Fe2.8-Na2 EDTA3.8g-B150Mn100-Znftul5 mg/100kgHp) usd as micro-elements in production of disease-free minitubers irrigating once every three weeks was the efficient way for miniatures production. It can improve the quality and quantity of minitubers.From experiment of microtubers dormancy breaking, It concluded :dormancy can be broken by QA3N Rs,2, 4-D and GA3liquid The highest sprouting ratio can respectively reached around 80%, 90%, 100%. The sprouting ratio was too low by GA3 liquid treating. The phenomenon of rotting potato was found in RS smokuig-Treating with needle and immersing in (2,4-D02mg+GAj2mg) ?L'1 liquid for 8hs was the best way for dormancy breaking of microtubers. The sprouting iatk) of mhrotubers can reach 100% and have no rotting potato by this way.