Distribution And Antioxidation Of Selenium Applied In Different Forms In Shoots Of Phyllostachys Violascens

Biofortification of Se-enriched shoots of Phyllostachys violascens was carried out culm lumeninjection of different forms of selenium, i.e., selenite, selenate and selenomethionine solution. Theobjective of the study was to investigate effects of the forms of selenium on accumulation, distributionand antioxidation of selenium in bamboo shoots, in order to provide the theoretical basis for seleniumbiofortification of bamboo shoots. The main results were as follows:1.Se contents in bamboo shoots were significantly increased by applying selenate orselenomethionine. Compared with control, the Se content of bamboo shoots treated with selenite,selenate, or selenomethionine increased35,99,269-fold, respectively. There were no significantdifferences of Se contents in the soil of stands of Phyllostachys violascens before and after the injectionof selenium, which indicated that culm lumen injection was environmentally friendly.2. Se-enriched bamboo shoots five selenium species, i.e., SeCys2, MeSeCys, Se (IV), SeMet andSe (VI), in which SeMet was the major forms. The contents of Se species were different amongdifferent Se treatments. Se (VI) in selenate-treated bamboo shoots was higher than that of the other twoSe treatments. The highest content of SeMet was in selenomethionine-treated bamboo shoots.3. In bamboo shoots, the main compounds containing selenium were proteins and polysaccharides.The selenium contents in proteins, polysaccharides and nucleic acids were increased for all treatments.The inorganic selenium of selenite was transformed more easily to organic form than that of selenate.4. The Se contents of water-soluble protein, alkali soluble protein, salt soluble protein andalcohol-protein in bamboo shoots were increased by all the Se treatments. The contents of proteinbinding Se was as the following order: alkali-soluble protein>water-soluble protein> alcohol-protein>salt-soluble protein. The contents of four proteins were different among the treatments, andalkali-soluble protein was the main component of protein.5. The molecular weight of four selenium proteins were9.9~97.7kDa,13.3~102.4kDa,10.1~84.7kDa and10.3~97.7kDa for control,selenite, selenate and selenomethionine treatment,respectively, and their protein bands were different. This indicated that Se treatments had effects ondistribution of selenium proteins in bamboo shoots. 6. Selenium proteins of three Se treatments had higher ABTS free radical scavenging and reducingpower than control. This indicated that three Se treatments could increase antioxidant of seleniumproteins in bamboo shoots.