Anatomic Observation Of Seed Coat Development In ’Jintian Huangjia Seedless’ Grape

In this study,‘Jintian Huangjia Seedless’ Grape which seed was abortive were used as materials, and the ‘Jintian Lianbaoshi’grape(nuclear) and ‘Crimson Seedless’ Grape as control. Single fruit weight, and seed weight,were investigated at different periods after anthesis and development process of seed coat was cytologically observated; On the basis of previous studies, the embryo rescue seedlings were subcultured. The cross triploid embryos with ‘Jintian Huangjia’ Seedless Grape as female parent and ‘Ju feng’ grape as male parent were rescued. The test conclusions were as follows.1. Seedless grape ovule weight of different varieties increased with the passage of time first, it began to decline when reached a peak, embryo weight. but the berries development model still showed a double ’S’ curve.2. The endosperm of ‘Crimson Seedless’ Grape developed full of the nucellar cells in45 days after pollinating. While the ovule weight reached the maximum value;Endosperm cells of ‘Jintian Huangjia’ Seedless Grape filled the entire embryo sac in45 days after pollination, and filled the nucellar tissue at 50 d after pollination.At the same time, the ovule weight reached a small peak.3. From 70 days to 100 days after pollination, the cross-sectional area of seed coat of‘Crimson Seedless’ Grape decreased, the vascular bundle shape changed obviously,Conduit and sieve tube integrated, and were not easily distinguishable. Most area of the cross section of the seed coat of ‘Jintian Huangjia’ Seedless was markedly thickened, cell size of vessels and sieve tubes increased. This feature become obvious at 80 d after pollination4. Triploid embryos of ‘Jintian Huangjia’ Seedless(diploid) ×’Kyoho grapes’ tetraploid were rescued. The effect was poor, germination rate was low, triploid plants was not obtained.