| Fecal samples of captive primates in the Fuzhou zoo were collected to study the status of gastrointestinal microecology. The real-time fluorescent quantitative PCR (qPCR) was used to analyze the contents of bifidobacterium, lactobacillus and E.coli in the samples.Through analyzing the contents of bacteria in feces, intestinal health and flora structure could be illustrated in the primates where they live on.Macaques were fed bifidus factor (stachyose, a kind of functional oligosaccharides) for 6 weeks to observe the influence of stachyose on gastrointestinal flora in macaques, and to analyze the mechanism of stachyose in adjusting intestinal flora. Blood samples were collected from macaques fed with stachyose and blood parameters were determined to explain the promotion metabolism of stachyose fed to macaques.(1) The results showed that the average number of bifidobacteria in macaques feces, papio Anubis feces, ring-tailed lemurs feces and cebus apellas feces were 4.5 X 107 CFU·g-1,7.9×107 CFU·g-1,1.9×108 CFU·g-1 and 1.4×108 CFU·g-1 respectively. The number of bifidobacteria in ring-tailed lemurs feces was significantly higher than the number of macaques (P<0.01), and the same as the cebus apellas feces (P<0.05). There were significant differences between youth body and the elderly body in the same species of macaques, papio Anubis and ring-tailed lemurs. And the average number of lactobacillus in macaques feces, papio Anubis feces, ring-tailed lemurs feces and cebus apellas feces were 4.1×106 CFU·g-1,4.8×106 CFU·g-1,9.3×106 CFU·g-1 and 9.3 X 106 CFU·g-1 respectively. There were significant differences between macaques and ring-tailed lemurs and cebus apellas(P<0.01),and between papio Anubis and ring-tailed lemurs and cebus apellas (P<0.05),and between macaques adult body and the elderly body (P<0.05).And the average number of E.coli in macaques feces, papio Anubis feces, ring-tailed lemurs feces and cebus apellas feces were 1.1×107 CFU·g-1,8.5×106 CFU·g-1,4.6×106 CFU·g-1 and 4.3× 106 CFU·g-1 respectively. There were significant differences between macaques and ring-tailed lemurs and cebus apellas(P<0.05),while no significant differences between different types of different age groups (P>0.05).(2) The caged primates were divided into four groups(contro, infancy,youth, and adult body group),and the quantities of bifidobacterium, lactobacillus, and e. coli in macaques faces were quantitative detected on the 6w stachyose feeding process.The results showed that the bifidobacterium growth trend of experimental groups was obvious in 1～2 w (P<0.01), different age groups were increased.The growth trend was reduced in 3-6w, and finally the contents could up to more than 109 CFU·g-1.The lactobacillus growth trend was slow during the test, but the different age groups increased 2～4 times in 0～6 w (P<0.05).The quantities of e. coli reduced significantly during the test (P<0.05).(3) The primates’ blood physiological and biochemical levels were compared and analyzed before and after the test. The results showed that the red blood cells, lymphocytes, adenosine deaminase, apolipoprotein, cholesterol, triglycerides, HDL cholesterol and blood calcium indicators had improved significantly (P< 0.05) after feeding stachyose. Stachyose could significantly affect the blood cholesterol and triglyceride metabolism of lipids in the body, and was good for blood calcium absorption.Therefore stachyose can improve the structure of intestinal flora, and promote the body absorb nutrients, improve the macaques’immune function and disease resistance. |
PDF Full Text Request