Expression Difference Of Genes In The Caragana Korshinskii Young Leaves Under Drought Stress By Different Electric Field Treatment On Seeds

The effects of different electric field conditions treatment on same kind of plant seeds are not identical. This paper is based on the early study:impact of electric field treatment on Caragana korshinskii (a kind of psammophytes) seeds under drought resistance. We selected the significantly promote field condition (the strength is 2kv/cm, the frequency is 15khz, the processing time is 5min, called ET1) and the significantly suppression field condition (the strength is 4kv/cm, the frequency is 13khz, the processing time is 15min, called ET2).The Caragana korshinskii seeds were treated by electric field and the seedling growth was under drought stress conditions, the expression difference of genes in the Caragana korshinskii young leaves are tested by DDRT-PCR. In order to compare the gene expression difference, young leave tissues were classified three samples include nature control (called CK), drought stress control (called SCK) and electric field treatment group (called ET1 and ET2). The seedling grew under drought stress using PEG-6000 to stimulate drought conditions for SCK、ET1 and ET2. The 3 anchor primers and 12 random primers were used to form 36 primer pairs, the DDRT-PCR was carried out 144 PCR reactions for the four kinds of samples. The cdna bands showed significant difference between four young leaves tissue samples. Compared with CK sample, SCK sample appeared 80 novel bands and disappeared 145 bands, ET1 sample appeared 115 novel bands and disappeared 130 bands and ET2 sample appeared 110 novel bands and disappeared 99 bands. Compared with SCK sample, ET1 sample appeared 158 bands and disappeared 103 bands and ET2 sample appeared 163 bands and disappeared 86 bands. Besides, compared with ET1 sample, ET2 sample appeared 106 bands and disappeared 83 bands.The results show that the impact of different electric field conditions in Caragana korshinskii seeds treatment on seedling leaves’gene expression under drought stress is not the same. Besides, significantly promote condition affects greater than the of significantly suppression field conditions. Our result provides experimental basis to analyze the biological mechanisms of drought resistance improvement induced by electric field treatment on plant seeds from the gene expression difference.