Study On The Effect Of Adsorption And Degradation Of 4 Kinds Of Mycotoxins

The adsorption efficiency for AFB1, ZEN, DON and FB1 were studied by mycotoxin absorbers that composed of montmorillonite and yeast cell wall in this study.Further more the adsorption effect of mycotoxins was studied by adding different proportion of mycotoxin absorbers and in different pH conditionis. Moreover, 5 strains that consistent with the list of varieties of fodder additive were selected to detect the degradationcapacities for mycotoxins.The excess mycotoxin absorbers composed of montmorillonite and yeast cell wall in a variety of different proportions were added to let mycotoxin standards were absorbed absolutely in the low mycotoxin concentration conditions. The mycotoxin detection experiment were performed by ELISA kit indicates that montmorillonite had obvious effect on AFB1 and FB1 in the methanol, however the absorption effect of ZEN was not obvious and had no adsorption effects of DON. The yeast cell wall showed no adsorption effects on AFB1,FB1, DON but showed absorption effect of ZEN although the absorption effect was not significantly.Different proportions of montmorillonite were added into the methanol contains the standards of AFB1 and FB1 to absorbing them. And the results indicate that with the increasing amount of montmorillonite, the mycotoxin absorption effect was enhanced. While after adding 0.5% montmorillonite, the adsorption rate of AFB1 and FB1 reached 51.93% and84.54% respectively under laboratory conditions. However, different phenomenon was observed for the corn meal contains AFB1 and FB1, the adsorption rate reached 72.64% after adding 0.5% montmorillonite but the adsorption effects of FB1 disappeared. Interestingly, the absorption effect was observed in corn meal soulutions after added 0.5% montmorillonite and the absorption effects was affected not obviously under different pH conditions.Fianlly, 5 strains were selected to perform degradation experiments of the material that composed of bran, peanut meal and corn germ meal contained AFB1, DON and ZEN mycotoxins. And the results showed that Bacillus licheniformis and Bacillus subtilis had thedegradation effect on AFB1, however no degradation effect was observed for other strains.Further tests indicate: mixed bacteria fermentation have priority than pure strain fermentation in the degradation of AFB1. Noteworthy, no degradation effect on DON and ZEN was observed by all the 5 strains.This study confirms that montmorillonite has obvious effect of adsorption on AFB1 under the liquid and the solid conditions, however no obvious adsorption effect was observed for other toxins. Meanwhile the yeast cell wall does not possess the ability to adsorb mycotoxins. And none of the 5 strains selected in this study possesses the capacity to degrade the mycotoxins in feed effectively.