| In this thesis,6 commercial (absorbent 1,2,3,4,5 and 6) and 3 lab prepared absorbents (P1, P2and P3) were evaluated for the adsorptive efficiency to aflatoxin B1 and zearalenone in simple isothermal, gastrointestinal fluids and in vivo trail. Meanwhile, a simple HPLC method to simultaneous determination of aflatoxin B1 and zearalenone in compound feed and its plant origin ingredients were verified in our lab.Exp.1:In vitro evaluation of adsorptive efficiency to aflatoxin and zearalenone.The protocol of simple isothermal adsorption was conducted as follow: when the densities of mycotoxins were 10μg/ml and the adsorbents were 1.5mg/ml, the adsorptive rates to aflatoxin B1 of 9 adsorbents were as follows,83.85%,42.62%,43.51%,75.09%, 63.49%,61.32%,64.46%,70.77% and 92.93%, the adsorption rates to zearalenone were as follows,23.45%,96.02%,20.01%,12.64%,13.38%,16.21%,56.67%,86.30% and 97.42%. In the gastric the small intestinal environment, the adsorption rates of 9 adsorbents to aflatoxin B1 were as follows,58.37%,12.58%,33.72%,76.94%,54.75%,42.97%,44.55%, 50.83% and 71.22%, the adsorption rates to zearalenone were as follows,95.34%,29.36%, 15.08%,41.02%,73.21%,76.15%,23.94%,86.67% and 87.09%. The protocol of small intestinal environment was conducted as follow: when the densities of mycotoxins were 10μg/ml and the adsorbents were 1.5 mg/ml, the adsorption rates of 9 adsorbents to aflatoxin B1 were as follows,85.94%,42.62%,47.06%,79.72%,66.04%,58.72%,58.67%,66.08% and 75.32%, the adsorption rates to zearalenone were as follows,66.48%,72.48%,69.38%, 29.63%,82.77%,86.22%,94.55%,96.32% and 94.38%.Exp.2:The feastibility verification of HPLC method to determination of aflatoxin Bi and zearalenone in compound animal feed and individual plant ingredientsThe HPLC parameters for determinating aflatoxin B1 and zearalenone were optimized. The recommended mycotoxin extracting solution was 90% acetonitrile, after purification by hexane and dichloromethane, the samples were detectived by HPLC, under this condition, aflatoxin B1 and zearalenone show good linearity in the range of 10ng·ml-1～5.0 ug·ml-1 and 8.0ng·ml-1～5.0μg·ml-1 respectively and correlation coefficients were 0.9992 and 0.9997. The detection limits are 10.05ng/ml and 7.74ng/ml. The rate of recoveries for aflatoxin B1 is 89.10%～110.3% and zearalenone is 98.7%～104.1%, relative standard deviation(RSD) for aflatoxin B1 is 0.59%～12.83%, zearalenone is 2.58%～12.72%.Exp.3:Chronic toxicity experiment with broiler30 replacement gilts (mean body weight at about 70kg) were selected, randomly assigned to 6 treatment groups, and 5 replicates per treatment with 1 sow per replicate. The 6 dietary treatments consisted of: basal diet, basal diet+adsorbent P3+moldy corn, basal diet+adsorbent 1+moldy corn, basal diet+moldy corn, basal diet+adsorbent P3, basal diet+adsorbent 1. Main results are obtained as follows:1) Moldy feed decreased the growth performance of gilts. However with the addition of mycotoxin adsorbent 1 or P3, these adverse effects are prevented.2) Moldy feed significantly increases the activity of GGT (P<0.05), the density of estrogen 2(P<0.05) and the luteinizing hormone (P<0.05) in serum, moldy corn can also affects other indicators, but not significantly (P>0.05). With the addition of mycotoxin adsorbent 1 or P3, these adverse effects are prevented.3) The residual of aflatoxin B1 and zearalenone in stool are 348.55ng·ml-1 2645.99ng·ml-1,322.10ng·ml-1 and 2628.76ng·ml-1,270.84ng·ml-1,1992.63ng·ml-1 in the 3 moldy corn groups, the other groups are no mycotoxins detected. In serum, only basic diet adds moldy corn group detected a 2.71ng·ml-1 zearalenone. |
PDF Full Text Request