Seeds Germination And Tissue Culture System Establishment Of Lysimachia Davurica L

Lysimachia davurica Ledeb. is a kind of Lysimachia. This plant owns favourable medicinal and ornamental values. At present, researches on it are very few. So, it is needed to explore the rapid propagation for mass medicine production and obtaining the methods of this germplasm resource conservation with seeds and steam and leaves of Lysimachia davurica L.A series of experiments were performed on seeds germination and tissue culture establishment. Study results are as follows:1. Seeds with dormancy characteristics couldn’t germinate without GA3soaking. Harvested at same time, seeds germinated better in4℃dry storage than20℃dry storage. Seeds germinated best in the condition of400mg.L-1GA3soaked for24h and nurtured temperature30℃in light, with optimum germination potential, germination ratio and germination coefficient45.37%,50.93%,2.67, respectively.2. Experiment results showed that75%alcohol soaked for30s and followed by0.1%HgCl2soaked for4min was the optimum explants disinfection of leaves;75%alcohol soaked for30s and followed by0.1%HgCl2soaked for10min was the optimum explants disinfection of steams. The optimum collecting time of steam was from May to June of the year. The optimum steam primary culture medium was MS+6-BA2.0mg-L-1+IBA0.5mg-L-1, with callus induction rate, axillary bud sprouting rate and axillary bud germination index100%,100%,5.3respectively. After cultured7days, stems and leaves began to induce callus. The best leaves callus induction were obtained on medium MS+NAA1.0mg-L-1+6-BA0.5mg-L-1and MS+NAA1.0mg-L"1+6-BA1.0mg-L-1with same callus ratios95.56%. Darkness culture could suppress lealves callus induction and reduced callus quality. Leaf blade base was the ideal leaf callus induction material. Leaf abaxial side down culture was better than adaxial side down culture for inducting callus.3. MS+6-BA2.5mg-L-1+NAA0.005mg-L-1was the best steam callus differentiation medium, with differentiation ratio and budding index83.33%,6.87respectively;6-BA (2.0-2.5) mg-L-1’+NAA0.2mg-L-1+MS had obtained the best steam callus proliferation. MS+6-BA1.0mg-L-1+NAA0.1mg-L-1was suitable for leaves callus differentiation, with differentiation ratio and budding index74.07%,4.56respectively;6-BA0.5mg-L-1+NAA0.1mg-L-1+MS had obtained the best leaf callus proliferation. MS+6-BA2.0mg-L-1+IBA0.5mg-L"1was most suitable for shoots proliferation and elongation.4. The optimum medium for rooting was1/2MS+IBA0.5mg.L-1+1g.L-1activated carbon, with maximum rooting rate, root number, root length:99.89%,5.35,9.44cm.97.78%of plantlets survived follwing acclimatization and tansforred to a potting cultivation substrate mixture(2:1, vermiculite:humus).