Studies On The Callus Induction And Plant Regeneration Of Prunus Armeniaca

Thesis Prunus armeniaca mature embryo explants,first filtered out of the mature embryo of apricot uses the best disinfectant in disinfection and sterilization time,And apricot, and multiple shoot induction, callus induction and seedling rooting culture medium and exogenous hormones effects comparisons, the type and proportion of filtering,Eventually filter out the best during the tissue culture medium and optimal hormone combinations,Initially established a mature embryo of apricot regeneration system. Provided the basis for the industrial production of Prunus armeniaca.Results showed that:(1) Filtered in the experiment is best for induction of callus of Prunus sibirica medium is MS medium,the best hormones for callus induction ratios for6-BA1.Omg/L2,4-D0.5mg/L, wild Apricot on the mature embryo culture medium of cultured callus since the8th day appears.(2) Prunus armeniaca selected the best of multiple shoot induction medium for MS, optimal hormone combination for NAA0.5mg/L、6-BA1.Omg/L,multiple shoot induction rate,45.83%,multiple shoot longer and the least amount of time,multiple shoot would begin to grow on the16th day of vaccination.(3) Prunus armeniaca seedlings and rooted in the process to elect the best medium for MS meedium,Seedling cultivation of which filtered out of the process is the most suitable medium for MS+6-BA0.5mg/L+NAA0.5mg/L+30g/L sucrose+agar,6g/L;Rooting poocess to filter out the most suitable forMS+IBA0.6mg/L+30g/L sucrose+agar6g/L, Average number of roots around4.86, And root growth and in good condition.