Callus Induction And Establishment Of Plant Regeneration System Of Dianthus Chinensis

Dianthus Chinensis is an important ornamental herbaceous flower in Dianthus due to its high ornamental value and economic value.Therefore,the propagation of its good varieties and the creation of new varieties is of great significance in landscaping in the future.In this study,seeds and different organs of D.Chinensis were used as materials to screen callus induction medium and regeneration medium.And then,the selected medium was tested by different species of Dianthus.GUS gene was transferred to the induced callus of D.Chinensis to be transiently expressed using agrobacterium-mediated method.The results are as follows:1.The media of MS+1 mg/L TDZ+4mg/L KT+1 mg/L NAA and 3 mg/L 6-BA+0.5 mg/L NAA were better in the callus induction of seeds.Their callus induction rates were 95.4%and 89.7%,respectively.The callus obtained could be directly differentiated into seedlings under light,with the regeneration rates of 100%and 80.04%,respectively.Thus,MS+1 mg/L TDZ+4mg/L KT+1 mg/L NAA was considered to be the optimal medium for callus induction and regeneration.2.The highest Callus induction rate among different organs in D.Chinensis was apical bud with 85.71%,then followed by 77.83%of petal,72.97%of leaf and 72%of stem segment.In the process of callus differentiation,only the apical bud and stem segment were successfully differentiated into clustered buds.Their germination rates were 67%and 55%,respectively.3.The results of callus induction and regeneration of carnation seeds showed that MS+1 mg/L TDZ+4mg/L KT+1 mg/L NAA was also the best medium for callus induction and regeneration of carnation.4.β-glucuronidase(GUS)gene was introduced into the callus of D.chinensis by Agrobacterium-mediated method.The staining showed that GUS gene could be transiently expressed in the callus.