| Armeniaca sibirica,which is a major economic tree species north region in China and ecological greening tree species,has wide applied and practical value.Its progenitive velosity is slow,the mount of seeds tremendously effected by the climate and have biennial bearing,which hamper popularization and application of good varieties.Using the plant tissue culture technology has the big Propagation coefficient,obtaines stable fine properties seedlings of armeniaca sibirica and solves a efficient path of reproductive issues.In this paper,leaves,seeds and stem tips as explants.Which studied the callus induction and differentiation of different explants,the system of callus regeneration plant was established,By differentiation of embryonic callus studied somatic embryogenesis,in order to obtaine somatic embryo for Armeniaca sibirica.The results are as follows:(1)The result shows that matured embryoes and stem tips respectively are the best explants for callus induction and somatic embryos.(2)The disinfection time increases for Seed,The infection rate and induction rate drop,processing seed used 75%alcohol for 30s and 2%NaCIO for 4 min,infection rate was 6.6%,but the induction rate was more than 90%,which was the best disinfection method;however The best disinfection effect was 75%alcohol for 30s 0.2%HgC12 for 6min and 2%NaCIO for 4 min for stem tips.(3)Callus induction rate of mature embryo was 100%,in MS + 6-BA(0.5 mg/L)+NAA(0.5 mg/L),When the concentrations of 6-BA and NAA are 2.0 mg/L and 0.2 mg/L,the callus differentiation rate up to 5.88;And the mature embryoes directly induce multiple shoots in light culture,when the combination of 6-BA(1.0 mg/L)and NAA(0.2 mg/L),the groth of bud plexus are strong,thick green leaves,grow well and differentiation rate was as high as 10.75,which was the best medium of mature embryo induce multiple shoot clumps;Finally cutting down buds induce root under the effect of NAA and IBA.when the combination of NAA and IBA,bud plexus of low rooting rate doesn’t even have root and a large number of callus in the base.separately using NAA,along with the concentration of NAA increasing,shoot clumps root rate also will increase,the rooting rate was the highest for 75%when the concentration of NAA was 0.5 mg/L.(4)Adding appendage such as hydrolyzed milk protein,casein hydrolysate and inositol in the test of embryonic callus proliferation,however callus proliferation rate was the highest 92.2%in the adding casein hydrolysate;The induction rate of embryonic callus was as high as 40%in the hormone combination of 6-BA(1.0 mg/L)and NAA(0.05 mg/L)as differential media of embryonic callus. |
