Effects Of CPPU On Callus Induction And Plant Regeneration Of Peach Rootstock(Prunus Persica)

A stable and efficient regeneration protocol was a pre-requisite for plant genetic transformation. In this study, sterile plantlets of the peach rootstock (Prunus persica) was used as experiment materials. On the basis of the exisiting research, firstly, paraffin sections of the callus induced from leaves was observed, potential-embryonic callus was determined in cell morphology; then effects of CPPU on callus induction and conservation and plant regeneration of peach rootstock was researched. The main results were as follows:1. The appropriate concertration of6-BA for wild peach in vitro conversation was0.2mg/L. The vitrification rate was decreased in this concertration.2. The appropriate CTK for adventitious shoot regeneration from leaves of wild peach was CPPU0.1mg/L. And when CPPU0.5mg/L combined with NAA0.1mg/L, the regeneration rate was16.67%.3. The appropriate medium for callus induction from leaves of wild peach was LP+CPPU0.5mg/L+NAA0.5mg/L+sucrose20.0g/L+agar6.0g/L, leaves of wild peach were transferred to light cultivation after21d dark treatment.The optimal PGR for callus induction from the petioles of wild peach was CPPU0.1mg/L combined with NAA0.5mg/L.4. The appropriate PGR for callus conservation of wild peach was CPPU0.1mg/L combined with NAA0.5mg/L. The calli were subcultured for every28d, last dark incubation and saved. The optimal medium for callus regeneration of wild peach was LP medium contained TDZ lmg/L and NAA0.5mg/L, in which maximal regeneration rate was3.33%.5. The appropriate medium for rooting of wild peach in vitro was1/2LP+IBA1.0mg/L+NAA0.1mg/L+AC0.5mg/L+sucrose20.0g/L+agar6.8g/L. The rooting percentage was up to77.78%, the mean of root number of every plantlet was1.06and average length was7.18cm.