The Roots Morphology Changes Of Artemisia Annua Induced By Arbuscular Mycorrhiza Fungi And Its Physiological Mechanism

The root morphology of plant would changed after symbiosised with arbuscular mycorrhizal fungi(AMF), but its mechanism was not known. In this paper, through pot experiment, a research was conducted to investigate the effects of inoculating AMF Glomous versiforme and G. mosseae on the changes of physiological mechanism of root morphology. In order to provide theoretical basis for the use of AMF to improve the yield and quality of medicinal plants by artificial cultivated. The results are as follows:G.mosseae and G.versiforme could form mycorrhizal with Artemisia annua L, and improved the root activity of Artemisia annua. With the forming of mycorrhiza, the root morphology was changed. Except the growth of100d, two fungi could promote Artemisia annua root elongation and thickening, increased root volume and dry weight at other stages; but the tip numbers and crossing numbers were not obvious.In addition to the growth of70d, the CAT, POD and PPO activity of inoculated plants root were higher than those in the control in other stages. But the different strains have different effects of different enzyme activity at the same period, and the effects of a kind of AMF for the same kind of enzyme activity were also different in the different periods.With prolonging growth period, both in leaves and roots of MDA content were had a reducing process from low to high. Except in132d, the G.versiforme can increase it in leaves of Artemisia annua at other times, and can increase it in roots at40d and100d. the G.mosseae can also increase the MDA content in roots,of Artemisia annua and can increase it in leaves in the70-132d.The IAA content of inoculated plants’ leaves gradually increased along with prolonging growth period, and reached peak value at132d, while it was first increased and then decreased in leaves of control plant. It was increased first and then decreased in roots of three treatment plants. As the extension of growth period, the ABA content in leaves of three different treatment had a descending trend, which relatively large decline of inoculated plants with G.mosseae,and it was increased in roots.With prolonging growth period, the content of CTK were increased gradually in root of plants inoculation with G.mosseae and control plants. It was first increased and then decreased in roots of plants inoculated with G.versiforme, and reached the highest at100d. The control of CTK content in plant leaves was first increased and then decreased, and then increased, while CTK content in leaves of the plant increased first and then decreased. The zeatin content basically increased first and then decreased in different treatments of Artemisia annua leaves and roots. Except for40d, G.mosseae and G.versiforme can increase it in leaves in other times; and G.versiforme also can increase it in roots of Artemisia annul among40-1OOd.With the extension of growth periods, the artemisinin of Artemisia annua showed changes from low to high and then decreased, it was70d for in inoculated plants to reach it’s maximum content while control plants is100d..The artemisinin content of inoculation treatment was significantly higher than that the control in the growth of70d and100d.