Research On The Roots And Root Exudates Alleopathy Of Atemisia Annua Symbiosed With Arbuscular Mycorrhizal Fungi

Under the symbiosic condition of arbuscular mycorrhizal fungi, the growth of plant was significantly promoted, but its mechanism wasn’t clear yet. In this paper, medicinal plant Artemisia annua was used as an experimental plant to study the effects which inoculating Glomous mosseae influnenced on the Artemisia annua roots, root exudates and its allepathy through pot culture and hydroponics, to discuss whether the promotion of arbuscular mycorrhizal fungi on plant growth is related to the changes of roots and root exudates or not. It aims to enrich the theory of arbuscular mycorrhiza and artificial cultivation of medicinal plants, and to innovate the cultivated technology of medicinal plants. The research results are as follows:Glomous mosseae and Artemisia annua roots had formed symbiotic mycorrhiza, it showed significantly promoting effects on the height, the dry weight of Artemisia annua plant when compared with control treatment.Inoculating Glomous mosseae contributed to the roots of Artemisia annua plant development, there existes significant differences between treatments on the total length, area, volume, tips and forks of plant roots.Compared with control treatment, inoculating Glomous mosseae enhanced root activity significantly.Hydroponics and substrate extraction methods were used to collect Artemisia annua root exudates, GC-MS identification results show that Artemisia annua root exudates from hydroponic solution were mostly consist of ketones, alcohols, hydrocarbons, phenols, acids, pathalic acid and its esters. Inoculating Glomous mosseae increased the constituent of root exudates; and changed the relative content. The Artemisia annua root exudates collected by substrate extracting have similar constituents between treatments, there were mainly pathalic aid and its esters, hydrocarbons, alcohols and esters in the compounds of root exudates collected by substrate extraction, the relative content of phthalic acid and its esters is the highest among the constituents.Compared with control treatment, inoculating Glomous mosseae decreased the content of soluble protein, soluble sugar and available amino acid in the Artemisia annua root exudates which collected by hydroponic. Howerer, the change trend was contrary in the Artemisia annua root exudates which collected by substrate extraction.Compared with control treatment, inoculating Glomous mosseae decreased the kind and content of organic acid in the Artemisia annua root exudates which collected by hydroponic. Nevertheless, the kind and content of organic acid was increased in the Artemisia annua root exudates of inoculated treatment, which collected by substrate extraction.Raphanus sativus, Lolium perenne and Cucumis sativus were used as receptor plants in this experiment, measured their seed germination and seedling growth with Artemisia annua root exudates, which collected by two methods combing with two concentrate ways. Rusults indicated that receptor plants were affected by the allelopathy of Artemisia annua root exudates, but the reactions were different among receptor plants.In addition, inoculated treatment mainly showed inhibiting effect on receptor plants.As a conclusion, arbuscular mycorrhizal fungi Glomous mosseae and Artemisia annua were in a good symbiotic relationship. Under the symbiosic condition of arbuscular mycorrhizal fungi, it contributed to increasing Artemisia annua plant biomass, to enhancing root activity and changing roots morphology. Inoculating Glomous mosseae has some influences on Artemisia annua root exudates. It can also affect the content of soluble protein, soluble sugar, available amino acid and organic acid in Artemisia annua root exudates. Besides, Artemisia annua root exudates mainly showed inhibition on the three receptor plants.