Research On Enthanol Preparation From The Residue Of Rhizome Of Kaempferia Galanga L. After Extraction

After the rhizome of Kaempferia galanga L. is extracted, a great amount of biomassresidue is left. The residue has a special smell and spicy taste, which is not palatable as animalfeed. However, if the residue is abandoned, not only the biomass is wasted, but also theenvironment will be polluted. In this thesis, the production of ethanol from the residue of therhizome of Kaempferia galanga L.(RKG) after extraction is studied, and a thin-layerchromatography-image analysis (TLC-IA) method is developed to assay the glucose for theprocess evaluation. Main contents include:(1) Analysis of the main components of RKG: The RKG powder is observed bymicroscopic and histochemical methods. The RKG powder contains mainly starch grains,followed by the cell wall and duct mainly composed of cellulose, accounting for43%to45%and27%to30%of the total residue, respectively..(2) Assay of glucose using TLC-IA: The stationary phase was silica gel plate modified by0.1mol/L Na2HPO3, the mobile phase was butanol-acetic acid-water (4:1:1), and thevisualized reagent was solution of aniline-diphenylamine in phosphoric acid and acetone. TheSmart phone was used to acquire the TLC chromatogram and perform image analysis toquantify the glucose.(3) Pretreatment processes of the production of ethanol from the RKG: The RKG wastreated by heating to boiling for5minutes with1%hydrochloric acid solution at thematerial-to-solution ratio of1:7. Next the mixture was adjusted to pH6.5and α-amylase wasadded at1ml per1L mixture and allowed to react for40minutes. Next when the pH of themixture was adjusted to4.5and the temperature was cooled to55℃, an appropriate amountof cellulase was added to the mixture to perform liquefaction. After treatment, the glucosecontent of the mixture was40g/L.(4) Ethanol fermentation process of the production of ethanol from the RKG:Simultaneous saccharification and fermentation was used. After72hours fermentation, thealcohol content was about7.0%(V/V). In this thesis, the production of ethanol using the RKG is studied. There is lack ofliterature in that subject. It is a novel work.