| The author take the SPF big ear white rabbit as the experimental animal,takes the immunology,the microbiology and the molecular biology as the main technical method. Through analysising and researching mannose-oligosaccharides;raising the experimental animal and observation of change of intestinal tract;As well as research of the intestinal tract microorganism multiplicity influence of the mannose-olig osaccharides to SPF big ear white rabbit.Deeply research the effect mechanism which to the oligosaccharide physics and chemistry nature and the mannose-oligosaccha rides promote animal growth,main resuLt as follows:1 Analysis and research of the mannose-oligosaccharideIn order to study the ingredient of mannose-oligosaccharides,obtains the sedimentation under different volume by useing the ethyl alcohol to gradually subside,mensurates 2% solution of each sedimentation under the OD value of 620nm,compares with the deoxidizing sugar standard curve,obtains 1:3,1:4,1:6,1:9 sedimentation the deoxidizing sugar content is 42.9μg,58.2μg,85.3μg,94.3μg.Then,separately carries on the thin steel plate chromatographic analysis to 2%solution of the experiments mannose-oligosaccharides,1:3, 1:4,1:6,1:9 sedimentation solutions of 2%,1:9 supernatant fluid,2%solution of the standard manna oligosaccharide as well as sugar Marker,carries on basic characterization to each components,used the liquid chromatography to analyze content of each sugars of 0.2% 1:3 supernatant fluid and 1:3 sedimentation,polysaccharide,two sugars,glucose,fructose, total sugar content respectively be(content account by g/kg).1:3 supernatant fluid:156.00, 3.36,12.52,2.61,174.49,1:3 sedimentation:182.88,0.70,3.98,0.50,188.06.2 The raising of experimental animal and the observation of intestinal tract changeIn order to study the mannose-oligosaccharides to the animal intestinal tract influence, selects the SPF big ear white rabbit which it's body weight for 300g+20.0 and it's has the homogeneity and same quality,carries on the experiment as the experimental animal.Divide the big ear white rabbit into 8 groups,every day will record its body weight and the quantity of feed.In the first day,7th day,14th day,21st day and the last day.execute and anatomize four big ears white rabbits respectively,take the jejunum,the ileum,the colon,the caecum and the rectum separately as well as among their content,preserved in -70℃.Flusheing the small intestine wall with PBS,collecting intestinal juices and preserving it in -20℃.The findings indicated that the influence of oligosaccharide to the big ear white rabbit's growth upgrowth is not obvious,but has the remarkable influence to the intestinal tract bacteria colony's quantity and the intestinal tract structure.3 Research of the intestinal tract microorganism multiplicity influence of the mannose- oligosac charides to SPF big ear white rabbitStudies relations of big ear white rabbit's intestinal tract microorganism influence of the mannose-oligosaccharides to the SPF.Abstract directly the total DNA from caecum content of big ears white rabbits which have been raisen for 28 day,PCR expands and obtains 16S rDNA fragment,carries on the DGGE analysis,simuLtaneously uses the ordinary PCR to carrie on connection transformation clone filtration,measures the sequence and compare.Result:Feeding on differently level is biggist to intestinal tract microorganism's influence of the big ears white rabbits,the lowest number of DGGE strip belt is 18,the highest is 37;8 groups of same strip belts are 3.It can Indicates that oligosaccharide has remarkable effection to animal intestinal tract bacteria colony's balance,directly influence bacteria colony quantity and composing. |
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