Studies On Tissue Culture Of Kaempferia Galanga And Kaempferia Parviflora

Kaempferia galanga L.is a dual-purpose plant for medicine and food.Its flowers look like butterflies and can also be used as potted plants.Kaempferia parviflora Wall.ex Baker is an important medicinal plant.It has beautiful leaves and can be used as indoor foliage and undergrowth plants.Kaempferia galanga and K.parviflora mainly rely on rhizome propagation with low propagation efficiency and slow speeds.Moreover,repeated stubble culture will lead to species degradation,cause diseases and insect pests,and therefore lead to a yield reduction.Thus,the present study used K.galanga and K.parviflora as experimental materials to study the effects of explants selection,disinfection technology and plant growth regulators on plant tissue culture.Our goals were to cultivate aseptic plantlets and to provide feasible technical methods and theoretical basis for the industrial production of K.galanga and K.parviflora.The main results were as follows:（1）The rhizome of K.galanga was used as explants.MS was used as the basic culture medium.Through three-step disinfection,75%alcohol for 60 s,0.1%Hg Cl₂ for3 min,sterile water for once,0.1%Hg Cl₂ for 4 min,sterile water for five times,the explants were inoculated to starting medium as MS+1.00 mg/L 6-BA+0.10 mg/L NAA+250.00 mg/L Timentin（p H 5.8）.The success rate of surface disinfection of explants was high,and the highest was 83.33%.Through the analysis of different plant growth regulators combination and concentrations of 6-BA,TDZ and NAA,the best medium for the multiplication and rooting for K.galanga was MS+3.00 mg/L 6-BA+0.75 mg/L TDZ（p H 5.8）.The proliferation coefficient was 5.20.The shoots were vigorous.Bud proliferation and rooting occurred at the same time.The rooting rate was100%.The roots were stronger and longer.Subsequnetly,the rooted plantlets were transplanted in peat:perlite with volume ratio 1:3 in a greenhouse and cultivated for30 d.the survival rate was 100%.After adaption,the plantlets were transplanted in the field,and the survival rate was 100%.The plantlets grew well and had no diseases and insect pests.（2）The rhizome of K.parviflora was used as explants.MS was used as basic medium.After 75%alcohol disinfection,sterile water washing once,0.1%Hg Cl₂disinfection 4 min,sterile water washing once,0.1%Hg Cl₂ disinfection 5 min,and sterile water washing,the explants were then inoculated to starting medium as MS+4.00 mg/L 6-BA+0.10 mg/L NAA+250.00 mg/L Timentin（p H 5.8）.The success rate was 76.88%.The medium of MS+10.00 mg/L 6-BA+1.00 mg/L TDZ+0.20 mg/L NAA（p H 5.8）was the best medium for the proliferation of K.parviflora,which could not only obtain a higher multiplication of 4.33.The shoots were vigorous.Bud proliferation and rooting occurred at the same time Rooting rate was 100%.The roots were slender and developed many fibrous roots.Then,the rooted plantlets were transplanted in peat:perlite with volume ratio 1:3 in a greenhouse and cultivated for30 d.The survival rate was 68.58%.（3）Using the rhizome thin section of aseptic plantlets of K.parviflora as explants,the effects of different combinations of 6-BA,TDZ and 2,4-D plant growth regulators on callus induction were studied by orthogonal design.The results showed that the best medium for callus induction was MS+3.00 mg/L 6-BA+1.00 mg/L TDZ+0.20 mg/L2,4-D（p H 5.8）.The best induction rate of the callus cultivated for 60 d was 64.29%.Because of the time,the experiments of callus differentiation and bud induction were not carried out.