| Microorganism is an important part of the marine ecosystem. The diversity and specificity of marine environments such as high pressure, high salt, low temperature, oligotrophic, low light and other marine ecological environment endowed marine bacteria with diversities on species, physiology, ecological function and metabolism. Isolation, identification and preservation of the microbial resources is not only the basis of promoting the development of life sciences and biotechnology,but also an important port of national economic and social sustainable development.On the basis of microbial resources of offshore consolidation, in this paper we focus on strains isolated from salt pond in Jimo and genomic research. Strain YCWB25 T was isolated from the salt pond in Jimo(0m,120.82°E,36.50°N), the 16 S r RNA sequence analysis showed that the homology of the strain was approximately95% relative to other acknowledged strains in Genbank. During the process of identification, a new strain was isolated. The new strain showed identical 16 S rRNA sequences compared to the former strain, but with obvious differences in phenotype.In the present study, these two strains was analyzed by the method of transcriptome for different analysis. The specific studies are as follows:1. Isolation and identification of Salinovum rubellum gen. nov., sp. nov.,YCWB25 T. An aerobic Gram-negative, oxidase-positive, catalase-positive, strain YCWB25 T was isolated from saltern pond in Jimo-Daqiao of Qingdao, east coast of China. Phylogenetic analysis based on 16 S rRNA gene sequences revealed that the strain YCWB25 T belonged to the Roseobacter clade of family Rhodobacteraceae, and was related to members of the genera Pseudooceanicola(94.26-95.54% similarity),Yangia(94.94%), and Maritimibacter(94.78%). However, the novel strain did form a robust, distinct clade any species of the Roseobacter clade. The DNA G+C content of strain YCWB25 T was 65.6 mol%. The predominant respiratory quinone of the strain YCWB25 T was UQ-10. The dominant cellular fatty acids were C18:1ω7c(62.76%),C16:0(13.08%), C19:0CYCLO ω8c(7.64%), which together acounted for 83.48 % of thetotal fatty acids. The phenotypic, chemotaxonomic and phylogenetic data indicated that strain YCWB25 T represents a novel genus and species, for which the name Rubellusioceanicola saltfieldensis gen. nov. was proposed, with strain YCWB25T(=LMG25394=NCCB100318) as the type strain.2. The de nove transcriptome sequencing on strain YCWB25 was carried out on the platform of Illumina HiSeq TM4000. 3026526600 nt bases were generated totally.In the results of assembly,3873 unigenes were detected, total length for unigenes was 2703969052 nt, average length was 903 nt. For function annotation analysis, we got 1806, 2093, 2219 unigenes which annotate to the KEGG, COG, GO database, respectively.3. The difference of RNA-seq between strain YCWB25 and YCWB25-2. Based on transcriptome sequencing, the two strains were researched on the gene function and metabolic pathways by the differentially expressed genes. Based on RPKM, a total of 6 up-regulated genes were detected, and 276 genes were down-regulated in YCWB25-2 vs YCWB25. In this differentially expressed genes, a total of 23 genes with GO annotated genes, there are six genes annotated to Function Orthology gene annotation information, 12 genes annotated to Process Orthology gene annotation information and five gene annotated to Component Orthology information. |
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