Shandong Province Part Of High Level Female Tennis Players During The Winter Training On Physical Function Of Adjusting Control

This article through to the Shandong province high level female tennis players during the winter training on hemoglobin, red cell count, hematocrit, serum creatine kinase and blood urea nitrogen of five physiological indexes monitoring, assessment and feedback method, on the women’s tennis team of Shandong province for the winter training process real-time monitoring, to professional athletes are training content monitoring and physical function of monitoring is to ensure that athletes have good physical fitness is an important means of. In order to further improve the athletic level, explore the tennis players during the winter training of physical function changes in the law, for the Shandong province tennis sports scientific training to provide more adequate practice and theory.Subjects:In women’s tennis team of Shandong province8line focus on athletes as the research object, in which5passengers for the international master athletes,3peopleSetting:EDTA and vacuum vessel, Beijing biological engineering high technology company CK detection reagent kit, measured at a wavelength of340nm, reaction temperature37C, in the37C, in the AG II Ststem biochemical analyzer for automatically testing work. Reagent configuration and storage building biological company production of blood urea nitrogen kit,20ml of buffer to blood urea reagent, shake to dissolve with blood urea nitrogen reagent, can be stable for14days (2-10C). The12edition of the SPSS statistical analysis software.Protocol:a) the blood processing and preservationThe present study every Monday morning6:20-6:50between fasting blood, prepare for the EDTA and vacuum vessel, vacuum tube pump4ml in whole blood, with the EDTA lml EDTA tube pumping blood, immediate3WBC, vacuum tube standing for30minutes, weight and balance, two to153000rpm centrifugal tube minutes divided and bleeding, cleaning and packaging, to-80degrees Celsius freezer to save test.b) serum computational enzyme activity determinationUsing the Beijing health in the high technology of biological engineering company CK detection reagent kit, measured at a wavelength of340nm, reaction temperature37C, in the AG II Ststem biochemical analyzer for automatically testing work c) blood urea nitrogen determinationReagent configuration and storage building biological company production of blood urea nitrogen kit,20ml of buffer to blood urea reagent, shake to dissolve with blood urea nitrogen reagent, can be stable for14days (2-10C). Test time usually arranged on Monday morning6:20-6:50uses two kinetic method, using the Beijing health in the high technology of biological engineering company Urea detection kit, the wavelength of340nm, reaction temperature37C, in the AG II Ststem biochemical analyzer for automatically testing work.d) hemoglobin, red cell count, hematocrit determinatioA fasting blood sample, EDTAK2anticoagulant, using three classification blood counting instrument determination (Sysmex KX-21N, Japan).Main Outcome Measures:Hemoglobin (HB), red blood cell count (RBC), hematocrit (HCT), serum creatine kinase (CK), blood urea nitrogen (BUN).Results:a)1, hemoglobin (HB) from the first week of training, hemoglobin started slightly decreased, but no significant difference. Third weeks to a minimum, fifth weeks, sixth Zhou Lve has picked up. Compared to the baseline values, training two, surrounded by hemoglobin has decreased significantly (P<0.05), three weeks of training aresignificantly decreased (P<0.01).Winter training period, and three weeks of training, training six weeks when the content of Hb were significantly higher (P<0.05).b) red blood cell count (RBC), along with the training week number increases, the number of red blood cells and existing decreased after increasing trend, but did not appear obvious changes. In the normal adult female red cell number range.c) the hematocrit (HCT), group and the experimental hematocrit did not change, and were in the normal range, each training before and after blood press numerical have no obvious difference, descriptions of experiments of nutrition factors on hematocrit changes did not significantly affect.d) serum creatine kinase (CK), compared to the baseline values,2,3weeks after training, creatine kinase values were significantly increased (P<0.01);2,3weeks of training in large quantity, to the athletes stimulation obviously, and3weeks of training, training4,5weeks have very significant decreased (P<0.01), training6weeks were significantly decreased at three weeks, according to the situation of timely feedback to the instructor a reduction in training volume, the creatine kinase values declined to normal levels,(P<0.05).e) blood urea nitrogen (BUN), blood urea in winter, in1.2weeks of training with little change, training3weeks after blood urea compared to the baseline values was significantly elevated (P<0.01); and3weeks of training, training for4weeks significantly reduced (P<0.01), training6week decreased significantly (P<0.05).Conclusionsa) In large amount of exercise training in the beginning stage, hemoglobin will assume the declining trend, but after the training the adjustment and the body of training adaptation, hemoglobin gradually rise.b) changes in hematocrit and red cell count changes is consistent, the changes can be used as evaluation index of endurance athletes, although there are fluctuations, but should be in the normal range.c) athletes’ serum creatine kinase values and blood urea showed decreased, the training week and quiet compared with significant difference.d) athletes during winter training reasonable nutrition, maintain physical function and complete the high quality training is important assure.