Based On The "evil Spleen Wet" Wet Investigate Each Factor On Rat Intestine Immune Factors And Intestinal Flora

TCM theory of correspondence between man and universe represented the holistic concept of Chinese medicine. External changes cause the body to respond physiologically and pathologically, which reflects the united relationship between human and nature,such as spleen being adverse to dampness. At present, studies more focus on observing clinical symptoms of spleen being adverse to dampness, less dig out the mechanism of it. Therefore, this study was designed to observe the change of IL-2and IL-10levels in jejunum, ileum and colon under humid and hot environment (long summer), according to TCM theory of Five-zang correspond to time. This study was also designed to observe the number of E.coil, LBA, BFB in jejunum, ileum and colon by controlling the relative humidity, in order to reveal the physiological mechanism of the spleen being adverse to dampness.1. ObjectiveThis study was designed to find the different number of IL-2and IL-10in jejunum, ileum and colon under follow climatic patterns, and reveal the principle that intestinal functions are adjusted with climatic change.In addition, this study is also trying to obverse the number of E.coil, LBA, BFB in jejunum, ileum and colon by controlling the relative humidity, and then to reveal the effect of humidity towards intestinal tract, thus to prove TCM theory of the spleen being adverse to dampness.2. Methods2.1Document researchesWe summarized the documents of the TCM theory of the spleen being adverse to dampness and intestinal microorganism and their experiments by documentary and logical analysis.2.2Experimental researches2.2.1The impacts of climate on intestinal immune factors(1) Group:In TCM theory, the climate contains five seasons, namely spring, summer, long summer, autumn and winter. The mice were divided into the control and pinealectomized groups, each of that had6mice, naturally fed for a month in each season. Finally, the levels of IL-2and IL-10in jejunum, ileum and colon were tested respectively.(2) Feed conditions:In natural daylight, the mice were fed on standard diet at (18±1)℃.(3) Experimental methods:Tested the levels of IL-2and IL-10by ELISA. 2.2.2The impacts of humidity on the numbers of E.coil, LBA and BFB in intestinal tract(1) Group:The mice were divided into normal temperature normal humidity environment (NNE), normal temperature high humidity environment (NHE), and normal temperature low humidity environment (NLE) group,6mice in each group.(2) Feed conditions:In natural daylight, the mice were fed on standard diet at (25±1)℃.The NNE group were raised at (60±5)%rh; the NLE group were at (30±5)%rh; the NHE group were at (90±5)%rh.(3) Experimental methods:After21days feed,5cm samples were taken out from jejunum, ileum and colon respectively, and then put the three samples in4ml normal saline. Finally test the number of E.coil, LBA and BFB in the three samples by using plate count method.3. Results3.1The impacts of climate on intestinal immune factorsSignificantly, the levels of IL-2and IL-10in jejunum were highest in long summer (P<0.01). Compared with those of spring, summer and autumn, the levels of IL-2and IL-10in ileum were obviously higher in long summer and winter respectively (P<0.01, or P<0.05). The levels of IL-2and IL-10in colon were much increased in winter, compared with those of spring, summer, long summer and autumn (P<0.01). The trends of IL-2and IL-10levels in intestinal tract of pinealectomized group were totally different from that of control group.3.2The impacts of humidity on the numbers of E.coil, LBA and BFB in intestinal tractThe numbers of jejunum and colon E.coil in NHE group were obviously higher than those of NNE group (P<0.01, or P<0.05). The number of jejunum LBA in NLE group was much increased, compared with that of NNE group (P<0.01). The numbers of jejunum, ileum and colon BFB were obviously higher in NLE group than those of NNE group (respectively P<0.001, P<0.01, P<0.01). The number of colon BFB in NLE group was higher, contrasted with that of NNE group (P<0.05).4. ConclusionIL-2and IL-10levels in jejunum, ileum and colon correspond with climatic patterns change. Especially in the climate of Long summer, IL-2and IL-10levels of jejunum and ileum were obviously higher, compared with those of spring, summer, autumn and winter. These revealed the TCM theories of the spleen correspond to Long summer and the spleen being adverse to dampness.Pineal gland took part in the rhythmic patterns of IL-2and IL-10levels in intestinal tract. This discovery made up for the traditional understanding of intestinal function mechanisms in contact with taking food, not with pineal gland.Humidity impacted on intestinal environment obviously. E.coil multiplied rapidly in high humidity, which was bad for body. Low humidity environment benefited LBA and BFB breed, which was is conducive to health. The sensitivities of jejunum, ileum and colon were different, and jejunum was more sensitive. This study, to an extent, revealed the TCM theories of the spleen being adverse to dampness.