Protective Effects And Mechanisms Of The Prescription Ⅰ Of Cooling Blood And Removing Stasis On Stagnated-Heat Syndrome Cerebral Ischemia

These years the morbidity of cerebrovascular disease tends to rise. Because of thehigh morbidity, mortality and relapse rate, cerebrovascular disease has become one of thethree biggest causes of the death of the human beings. According to the survey ofepidemiology, the number of patients who suffer from. cerebral ischemia stroke take up86％of the total number of patients who suffer from cerebrovascular diseases, thereforethe medical area and society pay close attention on how to prevent the cerebral ischemiacerebrovascular disease. However, in western medicine, doctors mainly use thrombolysisto cure the cerebral ischemia cerebrovascular disease, which has a lot of side effects andmay also cause complication, while Chinese medicine has the unique academicadvantages in prevention the disease. Professor Zhou Zhongying raised that "Mixedstasis-heat" is the main cause of cerebral ischemia cerebrovascular disease, based on hislong-term clinical practice, and he suggested that cooling blood and removing stasis isthe basic way to cure the disease.This thesis is going to use the prescription I of cooling blood and removing stasiswhich is formed by Padix Paeoniae Rubra and other herbs. The prescription has theeffectivenesses of cooling blood and removing stasis and calming wind and dredgingcollaterals, which can apply in cerebral ischemia stroke caused by mixed stasis-heat. Andthis thesis is going to talk about how to set the model which suffers from cerebralischemia caused by stagnated-heat syndrome in the hope to fit the clinical etiology andpathogenesis better. This thesis is to explain the effects and mechanisms of prescription Iof cooling blood and removing stasis from aspect of hemorheology, cytokine and so on.The thesis also try to provide the explanation of causes of the disease and theexperimental evidence of clinical medicine usage.The thesis is altogether 3 parts: Part 1: The set of the stagnated-heat syndrome cerebral ischemia model.Objective: To set the animal model which is combined disease and syndrome fit theclinical etiology and pathogenesis of cerebral ischemia better.Method: Inject LPS into health and maleness rats through vena caudalis twice every24 hours. Tow hours later, make the model of stagnated-heat syndrome cerebral ischemiaproduced by occlusion of the left middle cerebral artery with line. By observing thehemorheology, clotting time, neurological deficits, cerebral infarction, water content,cerebral index and histology of the model rats, the thesis is going to compare this modelwith the simple model of cerebral ischemia produced by occlusion of the left middlecerebral artery with line. And then we can see which model fit the clinical diseasestreatment better.Result: The hemorheology, clotting time, neurological deficits, cerebral infarction,water content, cerebral index and histology of the model of stagnated-heat syndromecerebral ischemia are more obvious than the simple model of cerebral ischemia producedby occlusion of the left middle cerebral artery with line, and all of these indexes showthat the stagnated-heat syndrome cerebral ischemia model hears strong resemblance topatients’ clinical manifestations.Part 2: How the prescription I of cooling blood and removing stasis protect thestagnated-heat syndrome cerebral ischemia rats.Objective: To observe the prescription I of cooling blood and removing stasis protectthe stagnated-heat syndrome cerebral ischemia rats.Method: Based on the prepared the stagnated-heat syndrome animal model make themodel of stagnated- heat syndrome cerebral ischemia produced by occlusion of the leftmiddle cerebral artery with line. To observe how the prescription I of cooling blood andremoving stasis affects the stagnated-heat syndrome cerebral ischemia rats in bloodhemorheology, cerebral edema, brain homogenate biochemistry and IL-1, IL-2 and NO,NOS in blood serum.Result: The prescription I of cooling blood and removing stasis can improve thesituation of the stagnated-heat syndrome cerebral ischemia rats in the change ofhemorheology, cerebral edema caused by stagnated-heat syndrome cerebral ischemia.And it also controls the production of NO, NOS in blood serum and releases the IL1,IL-2 in blood serum. Part 3: How the prescription I of cooling blood and removing stasis affects bloodvessels and what is its mechanism of action.Objective: Research how the prescription I of cooling blood and removing stasisaffects blood vessels and what is its mechanism of action.Method: Through the research of basilar artery learn the effect of prescription I ofcooling blood and removing stasis on contractile response of brain vessels, the release ofcell LDH and use 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyterazolium bromide（MTT）assay to measure of protection effect of prescription I of cooling blood and removingstasis on the anoxic and anoxemic injury model of EVC304.Result: The prescription I of cooling blood and removing stasis can rivalry tonecontraction caused by desoxyepinephrine; It can cut down on the leakage of LDH; It cancontrol the endothelial cells injury caused by H₂O₂ and Na₂S₂O₄. All of these show thatthe prescription I of cooling blood and removing stasis can rivalry the anoxic andanoxemic injury of endothelial cell.