The Treatment Effect And The Protective Mechanism Of The Prescription Of Cooling Blood And Removing Stasis And Its Demolition Permit On The Stagnated-Heat Syndrome Of Cerebral Ischemia

Research purposesIn recent years, the "stagnated-heat" theory has been listed as National Key Basic Research Development Program - 973 projects and systematic clinical studies are ongoing. In this study, the experimental research of blood stasis and heat of cerebral stroke carried out , and molecular biology from the scientific content of the doctrine are discussed.Research Methods1. To establish a rat model of blood stasis and heat of cerebral ischemia, and observate the changes of brain index ,the impact of brain edema and the change targets of the hemorheologyof the model after administration.2. Application of ELISA method in serum IL-4, IL-6 and HSP70, TNF-a and other inflammatory factors, as well as the content of NO and NOS activity.3. Using MTT and detection of LDH release to observe the effects of the serum containing of the the prescription of cooling blood and removing stasis on the hypoxia-ischemia cell.The results1. The research findings show: The whole prescription than its disassembled prescriptions with a significant protective effect, not only can alleviate the situation of brain edema, and blood viscosity, such as changes in blood rheology, but also be able to significantly improved tPA, PAI-1 andβ-TG, PF4, such as blood coagulation and fibrinolysis in relevant factors. At the same time, like IL-6, TNF-α, NO, NOS, HSP70, such as inflammatory cytokines and high expression of active substances, but also reveals the " stagnated-heat "syndrome of rats of cerebral ischemia related to the molecular mechanism of certification.2. After the normal HUVEC cells were given the original high-dose drug-containing serum, the cell supernatant of LDH of the damaged cells were significantly reduced, and cells were reduced significantly damage.