| ObjectiveTo observe the effects of trimetazidine on rats with heart failure, which include cardiac function in normal circumstances and further clarify the role of trimetazidine for the treatment of CHF, and to determine the IL-12and IL-18levels of the blood of rats with CHF to explore trimetazidine anti-CHF effects mechanism on CHF rats.MethodsApplication of abdominal aortic coarctation chronic heart failure model, then separate the animals into heart failure control group of13, and heart failure song trimetazidine intervention group of13through random-number-table way, in which heart failure trimetazidine intervention group expose with trimetazidine. In addition,10healthy peers rats as sham operation group (SH), determine the degree of cardiac hypertrophy, Left ventricular mass, left ventricular diastolic diameter (LVDd), left ventricular diameter systolic (LVDs), fractional shortening (FS), and left ventricular ejection fraction (EF) through echocardiography and surgery. After taking the blood was centrifuged and the supernatant saved from the ventricle, IL-12and IL-18was measured by enzyme-linked immunosorbent assay (ELISA).Results1. After trimetazidine administered rats with CHF in4weeks to exame cardiac function by cardiac ultrasoun, the rats was recorded of cardiac function index which contained left ventricular diastolic diameter (LVDd), left ventricular systolic internal diameter (LVDs), fractional shortening (FS), left ventricular ejection fraction (EF). The results showed that diastolic left ventricular internal diameter (LVDd), systolic left ventricular internal diameter (LVDs) highest value, ejection fraction (EF), and fractional shortening (FS) value on CHF-C group was the lowest compared with the others, and the difference was statistically significant between the groups (P<0.05). The left ventricular diastolic diameter (LVDd), systolic left ventricular internal diameter (LVDs) value of the minimum, ejection fraction (EF), and fractional shortening (FS) value on SH group was the highest compared with the other groups, among the groups, and the difference was self-evident significant (P<0.05). The difference of the Left ventricular diastolic diameter (LVDd), systolic left ventricular internal diameter (LVDs), ejection fraction (EF), and fractional shortening (FS) comparison value on CHF-S group between groups was not statistically significant (P>0.05).2. Ventricular remodeling after administration of each comparison group compared with the SH group, CHF-C group and CHF-S group to reduce body weight in the intervention group (P<0.05, P<0.05). CHF-S group in the intervention group compared with CHF-C group increased body weight (P<0.05).Left ventricular mass weight of the three groups was not statistically significant direct, left ventricular mass intervention group of CHF-C and CHF-S were lower than SH group.LVE/BW of left ventricular mass index CHF-S group was between SH group and CHF-C group. Compared with SH group, CHF-C and CHF-S group were lower, and the difference was statistically significant (P<0.01, P<0.01). The difference of the LVE/BW of CHF-S group compared with the CHF-C group was statistically significant (P<0.05).3. Myocardial tissue of each group rats after administration observed under an inverted microscope by HE staining. The results showed that myocardial cell size and structure were normal alignment rules. Cytoplasmic staining was uniform. The number of nuclei and staining were normal on SH groups. On CHF-C group, myocardial cell swelling was ill-defined, and a small number of nuclei. Chromatin shallow lightly stained cytoplasm partial cavitation. Room filled with a large number of red blood cells or muscle fibers neutrophil infiltration. Morphological changes CHF-S group than CHF-C group had improved to varying degrees, whichy contained the cells was slightly swollen, the gap increased slightly, and a small amount of blood cells on CHF-S group.4. After the experiment each group comparing rat myocardio cells cross-sectional area, the minimum of cross-sectional area with the SH group compared CHF-C and CHF-S, the difference was apparent significant (P<0.01,). By contrast CHF-C with CHF-S, the difference was apparent significant(P<0.01).5. After the experiment each group comparing IL-12and IL-18, the minimum value of IL-12with the SH group compared CHF-C and CHF-S, the difference was apparent significant (P<0.01, P<0.05). By contrast CHF-C with CHF-S, the difference was apparent significant(P<0.05). Contrast to IL-18in each group, IL-18of the CHF-C was the highest value. With CHF-S, the difference was apparent significant (P<0.01).Conclusions1IL-12and IL-18of CHF rats variation suggested that the production of IL-12and IL-18synthesis synergy led to enhanced cardiac reactions, promoting the development of heart failure2.,Trimetazidine through correcting the imbalance of cytokines IL-12and IL-18significantly improve the symptoms of heart failure in rats, followed by large-scale clinical trials still need to be given evidence-based science evidence. |
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