The Abnormal Expression Of MiR-21and MiR-218in Cervical Cancer And Precancerous Tissues And The Relations With Clinical Pathology

Objective: To investigate the expression of miR-21and miR-218incervical cancer tissues and precancerous lesions and the relations with high-risktype HPV infection and the clinical pathological features of cervical cancer.Methods:miRNA microarray was applied to screen the differential expression ofmiRNA in cervical cancer and cervical intraepithelial neoplasia lesions.Real-time quantification RT-PCR was used to validate the expression of miR-21and miR-218in108cervical tissues. Results:(1)Differential miRNAs wereidentified in cervical cancer and high-grade squamous intraepithelial lesion(HSIL).MiR-218was most down-regulated and miR-21was mostup-regulated.Meanwhile, real-time quantification RT-PCR result accorded withmiRNA microarray result.(2)The positive rates of high-risk type HPV infectionin cervical cancer and high-grade squamous intraepithelial lesion are more than72%,in which，the main HPV infection is HPV-16，the second is HPV-18and thethird HPV-45.There is no obvious correlation between High-risk type HPV infection and the pathological types of cervical cancer.(3)The expression ofmiR-21is associated with the tissue-differentiation and the high-risk type HPVinfection of cervical cancer,but it has no obvious correlation with some clinicalpathological characteristics of cervical cancer, such as FIGO staging, types ofpathology, lymph node metastasis and vascular invasion.(4)The expression ofmiR-218is associated with the high-risk type HPV infection of cervicalcancer,but it has no obvious correlation with some clinical pathologicalcharacteristics of cervical cancer, such as FIGO staging,types of pathology,tissue-differentiation, lymph node metastasis and vascular invasion.Conclusion:miR-21is aberrant highly expressed in cervical cancer,which isassociated with the high-risk type HPV infection and the tissue-differentiation ofcervical cancer.miR-218is aberrant lowly expressed in cervical cancer andcervical precancerous tissues,which is associated with the high-risk type HPVinfection.MiR-21and miR-218may play an important role in cervical cancerand precancerous lesions.