| Humulus Lupulus is a long history medicine food homology plant which has advantageslike convenient source and safety. Humulus Lupulus mainly contained many chemicalconstituents such as resinae, flavonoids, naphtha, bilineurine, aminophenol, tannin and corasefiber, etc. The main constituents of hops are flavonoids, Flavonoids is polyphenoliccompound ubiquitous in plants, also the natural growth process plants produce a secondarymetabolite produced. Flavonoids have antioxidant, anti-radiation, anti-aging, anti-fatigue,antibacterial and hormones-regulating-function. Therefore, Humulus lupulus has the activesuch as estrogen like effects, anti-radiation, antioxidant, antimicrobial, antitumor andsedative activity, etc.The recovery process of the radiation damage is very long, how to reduce theradiation damage, and protect the health of the people, especially the radiation protection fortumor patients receive clinically radiation therapy and people who occupationally exposed toradiation. It is very important to development anti-radiation medicine which is safe, stableand recovery promoted. We assess the effect of Humulus Lupulus on anti-radiation on thebasic of separation and purification of Flavonoids.First of all, separated and purificated Flavonoids from Humulus Lupulus by ethanolextraction and macroporous resin adsorption. Then technological parameters were optimizedon the basis of the content and yield of flavonoids. The results showed that the optimumseparation and purification process of flavonoids of Humulus Lupulus is40%ethanolextraction, using FL-3macroporous resin, the pH value is4, in20%ethanol conditions ofstatic adsorption, then in the pH value is7,60%ethanol conditions of elution. Usingthis process the content of flavonoids in the samples is70.6%, the total yield comes up to70.2%.Secondly, we study the effect of Humulus Lupulus on radiate injury mice through invivo experiment which detect WBC, BMNC, the content of DNA of bone marrow cells,organ coefficient (thymus, spleen, liver), the activity of GSH-Px and SOD and the content ofMDA in whole blood and liver.After irradiation, organ coefficients of spleen and thymus of the radiation group weresignificantly lower than normal groups (P<0.05). Compared with normal group, organcoefficients of spleen and thymus in low, medium and high dose groups were significantly lower (P<0.05), and the high group was the lowest. Compared with the normal group, organcoefficient of liver in radiation groups, were significantly lower (P<0.05), especially the highgroup.Compared with the normal group, WBC, BMNC, the content of DNA of bone marrowcells in radiation group, were significantly lower(P<0.05); Compared with radiation group,WBC, BMNC, the content of DNA of bone marrow cells in low, medium and high dosegroups were significantly higher (P<0.05), especially high dose group.The content of whole blood and liver MDA of rats in radiation group were significantlyhigher compared with normal group (P<0.05); Compared with the radiation group, thecontent of whole blood and liver MDA of rats from Low, medium and high dose groups weresignificantly lower (P<0.05), the difference between medium and high dose group of livertissue was significant (P<0.05). Compared with normal group the activities of whole bloodand liver SOD and GSH-Px of rats from radiation group was significantly lower (P<0.05);Compared with the radiation group, the activities of whole blood and liver SOD and GSH-Pxof rats from low, medium and high dose groups were significantly higher, and the activities ofwhole blood and liver SOD difference between medium and high dose group of wassignificance (P<0.05). The activities of whole blood and liver GSH-Px difference among low,medium and high dose groups were significance (P<0.05).The results show that ethanol extraction and macroporous resin adsorption methodachieve the goal that separation and purification of Flavonoids from Humulus Lupulus, andflavonoids of Humulus Lupulus produced by this process can protect mice from radiationinjury. |
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