Experimental Research On IL-1β, IL-6and PGE2in Acute Soft Tissue Injury Rats By Shangtongning Liniment

Shangtongning liniment comes from the treatment of acute soft tissue injury Tujiafolk prescription, and combines experiences of Tujia local doctors, according to thebasic pathogenesis of qi stagnation and blood stasis, it is developed from thelong-term consolidation and selection, Shangtongning liniment has efficacy ofactiviating blood, dissolving stasis, analgesia and easy to use. For the treatment ofacute soft tissue injury, has been in clinical use for many years.Objective: Through the establishment of an experimental model of acute softtissue injury in rats, to observe injury symptoms index, damaged muscle tissuemorphology, serum IL-1β, IL-6, PGE2levels and to study Shangtongning liniment’stherapeutic effect and mechanism of acute soft tissue injury, for the clinical use of thedrug to provide a scientific experimental basis.Methods: Through heavy hit for several times on rat，s left leg in the same place，60male SD rats are subjected to acute soft tissue injury divided into four groups: thenormal control group, the negative control group (the model control group)，thepositive control group(safflower oil group)，the experimental group(Shangtongningliniment group).After successfully establishing model of experimental animals，therats were sacrificed on the first day, the fourth day and the seventh day. The effects ofshangtongning liniment were observed on the local damage symptoms，damagedmuscle tissue morphology, serum IL-1β, IL-6and PGE2levels.Results: Damage symptoms indices：Shangtongning liniment group and saffloweroil group could Improve the Symptoms of swelling, bruising, dysfunction and so on.there are significant differences compared with the model group(P<0.05，P<0.01).Muscle tissue morphology：Shangtongning liniment group and safflower oil group cansignificantly reduce damage local soft tissue inflammation and promote the repair ofmuscle fibers. The serum IL-1β, IL-6and PGE2levels in model control group aresignificantly higher than the normal control group;Shangtongning liniment groupcould significantly inhibit IL-1β, PGE2and IL-6increased on the first day, the fourthday and the seventh day. Compared with the safflower oil group, it has no significantdifference in inhibition of IL-1β (P>0.05), and has significant differences ininhibitions of IL-6and PGE2(P<0.05); Compared with normal control group andmodel control group, Shangtongning liniment group and safflower oil group havesignificant differences(P<0.05，P<0.01).Conclusion: Shangtongning liniment has efficacy of activiating blood dissolving stasis and analgesia,, can significantly improve acute soft tissue injury swelling,bruising, pain and other symptoms, reduced IL-1β, IL-6, PGE2levels, improvepathological tissue damage, and its therapeutic mechanism is related to inhibition ofserum IL-1β, IL-6and PGE2.