Screening The Effective Component Group From Alisma Group

Obiective: To abstract and separate effective components from ansma and auacrvioder o’ Golaer. alisma soup.and to compatibitity. the every components. Opservec pharmacodynamk effects of each component grouo of anu-endlymphatic to select the best component group ot ann.-endlvmphatic.Providing the Ideas to treatment the modern diseases b\ancient prescription.and providing the research foundation to determine the active ingredient in the compound and develop the new drugs of anti-end lymphatic.Method. The volatile oil of atractviodes is obtained bv steam distillation and identified as component A.The polysaccharides from atractylodes is obtained by water-extract and identified as component B. With integrated indicators atractylodes extraction rate and the rate of atracrylodes polysaccharide as indicator.study medicine granularity, add water, extraction time three factors.every factor sets three levels.designing the orthogonal test to optimize the best extraction technology. The component group of alisma is obtained by the method of water extracts.Then the component group is separated by water extraction and alcohol precipitation.the part of alcohol is component C:the pan of precipitation is component D. The extraction conditions were optimized by L9(34) orthogonal test.with the solvent volume.extraction time and extraction times as the influencing factors, even-factor sets three ieveis.the extraction rate of triterpenoids from alisma were used as the evaluation indexes. Compatibility of the components for composing the component group of A+C、A+D、 A+B+C、A-B-D、A+C+D、B+C+D、A+B+C+D. Observation of each component group of the anu-endoiymphatic guinea pig model of water lost influence.Results: According to statistical software analysis results that there are significant difference in the impact factor A. there are difference in the impact factor C for each experiment and same in the impact factor B for each experiment in the process of extraction of atractvlodes. There are differences in the impact factor A and C for each experiment and same in the impact factor B for each experiment in the process of extraction of alisma.In the pharmacodynamic experiments, there are differences between the mode! group and the comparison group in each group is blank group.aiisma soup group.and the group of B+D、 A+B+C、A+B+D、 A+C+D、A+B+C+D Conclusion:The factors affect each experiment as A> C> B in the process of effective component extraction of atracryiodes. B almost no effect on the extraction results. The faetor A maximum of k3. the factor A level3should be selecred.namely AB. B factor less imnact on the results, according to the mos; savings, convenience scheme seleeuon, select Bl: The factor C maximum of k2. the factor C level2should be selected.namet C2The optimal conditions for the effective components atractviodes ss A3B1C2That medicine atractviodes40mesh particle size, adding six times with water and extracted five hours. By the results of the stability experiment shows that the optimal parameters with good reproducibility process stability The factors affect each experimem as A> C> B in the process of effective component extraction of alisma. B almost no effect on the extraction results. The factor A maximum of k3the factor A level3should be selected.namelv A3. B factor less impact on the results, according to the most savings, convenience scheme selection, select Bl; The factor C maximum of k3, the factor C level3should be selected.nameiv C3. The opumal conditions for the effective components alisma is A3B1C3that each extraction1.5h. add6times with water and extracted three times. By the results of the stability experiment shows that the optimal parameters with good reproducibilitv. process stability. By a previous study showed that Alisma soup has good resistance endolymphatic role in this project is consistent with the experimental results There are differences between the model group and the comparison group in each group is blank, group.alisma soup group.and the group of B-D. A+B+C A+B+D, A+C+D, A+B+C+D that these compatibility groups have anti endolymphatie role and the model copy is successful. Further analysis of the mean and the number of guinea pigs in endolymphatic can be found that the best group should be A+B+C+D group, the mean of A+B+C+D group is close to control group, and the endolymphatic guinea pig quantity is less.