Studies On The Pharmacy Of The Effective Anti-inflammatory And Analgesic Component From Chaenomeles Speciosa

In order to research the optimum technics of producing glycosides of chaenomeles speciosa(GCS)-the effective anti-inflammatory and analgesic component of chaenomeles speciosa, and to establish the quality standard, the pharmacodynamics experiments of three extracts from chaenomeles speciosa were studied as the parameter of anti-inflammatory and analgesic effect. Meanwhile,different conditions for extracting GCS were analyzed through the orthogonal design experiments with analysis of variance.The technical conditions and parameters of GCS's illustration and purification with macroporus adsorptive resin were investigated. The quality standards of GCS were established.To compare the anti-inflammatory and analgesic effects of the total extracts of chaenomeles speciosa (TECS), GCS and non-glycosides of chenomeles speciosa (NGCS),the ear swelling in mice with xylene was used. The anti-inflammatory effects of three extracts from chaenomeles speciosa were observed, hot-plate test was used to assess pain threshold in mice, and observed the analgesic effects of three extracts from chaenomeles speciosa. GCS 60,120,240mg kg-1 ig significantly inhibited the acute imflammation induced by xylene,and demonstrated the dose-related inhibitory effect, NGCS 200,400mg . kg-1 had no inhibitory effect. When GCS 120,240mg . kg-1 administered intragastrically , the pain threshold was risen remarkably in mice,and the analgesic effect was dependent on the dosage. GCS had the effect of anti-inflammation and analgesia. GCS is the effective component of anti-inflammation and analgesia from chaenomeles speciosa.The optimum preparation process of GCS was investigated by the orthogonal design as the GCS content index, which was determined by colormetric method. The optimum alcohol-extracting factors were 8 times of 80% alcohol, refluxing and extracting for Ih, for 3 times.The technical parameters of GCS's purification with macroporous adsorptive resin were investigated, and the quality control system was established. This technique was a good method to purify GCS.The water solution of the optimum alcohol-extracts was absorbed for Ih with a column of macroporous resin(l.5cm 16cm) and the resin was washed with 100ml of distilled water,then washed with 100ml of 20% alcohol, at last the GCS was eluted from macroporous resin with 100ml of 50% alcohol.The Chemical indentification and TCL of GCS were carried out. GCS was determined by colormetric method. The average recovery was 99.80%,and RSD was 1.82%. This method was simple, feasible and could be repeated. The method could be used to control the quality.The whole of the studies got the best technics of producing GCS, which purity exceeded 50%, and established the quality control standard. It will be the foundation for deeper development and research on GCS and the preparation,and supply the theoretical reference for GCS industrial production.