Expression Of Foxp3and IL─10in The First-degree Relatives Of The Patients With Gastric Cancer Before And After Eradication Of Helicobacter Pylori

Objective: We detected expression of Foxp3and IL-10before and afterHelicobacter pylori infection eradicated in the first-degree relatives of thepatients with gastric cancer, and tried to identify the role of HelicobacterPylori in gastric cancer, based on which we may provide some usefulevidences for Helicobacter Pylori eradication of patients with family historyof gastric cancer.Methods:20first-degree relatives of the gastric cancer with Hp(+) wereselected as the experimental group,and20first-degree relatives of the gastriccancer with Hp(),and20primary relatives of non-gastric cancer patientswith Hp(+), and20primary relatives of non-gastric cancer patients with Hp()were selected as control groups,all patients were non-gastric cancerousdetected by biopsy.Immunohistochemistry was used to detect expression ofFoxp3and IL-10genes in gastric mucosa of experimental and control groupsand experimental group with Hp eradicated.Results:1.The expression of Foxp3in the first-degree relatives of gastric cancerwith Hp(+) was55%,higher than10%of the first-degree relatives of gastriccancer with Hp(),the difference was statistically significant(2=9.231，p=0.003＜0.05);the expression of Foxp3in the first-degree relatives ofgastric cancer with Hp(+) was higher than40%of the primary relatives ofnon-gastric cancer patients with Hp(+),but the difference was not statistically significant(2=0.902，p=0.264＞0.05).The expression of Foxp3in the primary relatives of non-gastric cancerpatients with Hp(+) was40%,higher than5%of the primary relatives ofnon-gastric cancer patients with Hp(),the difference was statisticallysignificant(2=7.025，p=0.010＜0.05).After eradication of Helicobacter Pylori,the expression of Foxp3decreased by40%in the first-degree relatives of gastric cancer with Hp(+),thedifference was statistically significant(2=7.003，p=0.009＜0.05).2. The expression of IL-10in the first-degree relatives of gastric cancerwith Hp(+) was65%,higher than25%of the first-degree relatives of gastriccancer Hp(), the difference was statistically significant(2=6.465，p=0.012＜0.05;the expression of IL-10in the first-degree relatives of gastric cancerwith Hp(+) was higher than45%the primary relatives of non-gastric cancerpatients with Hp(+),the difference was not statistically significant(2=1.616，p=0.170＞0.05);The expression of IL-10in the primary relatives ofnon-gastric cancer patients with Hp(+) was45%,higher than15%of theprimary relatives of non-gastric cancer patients with Hp(),the difference wasstatistically significant(2=10.417，p=0.002＜0.05).After eradication of Helicobacter Pylori,the expression of IL-10decreased by45%in the first-degree relatives of gastric cancer with Hp(+),the difference was statistically significant(2=8.286，p=0.005＜0.05).3.In the first-degree relatives of gastric cancer with Hp(+) group,therewas a positive correlation between Foxp3and IL-10(r=0.591，p=0.006<0.01).Conclusions:1.Via up-regulating expression of Foxp3and IL-10of gastric mucosa,Hp infection inhibits normal immune response and clearance of Hp from thebody,and then leads to sustained infection,thus initiating formation anddevelopment of gastric cancer.2.Higher expression of Foxp3and IL-10in first-degree relatives of gastriccancer with Helicobacter pylori infection might be beneficial to formation ofthe micro-environment for immune escape of gastric cancer.3.Eradication of Helicobacter pylori infection decreased the expression ofFoxp3and IL-10in the first-degree relatives of gastric cancer,which restorednormal immune response and surveillance against the gastric cancer,and thendecreased the risk of gastric cancer.