Experimental Study On The Improvement Sleep By Anshenning Syrup

Objective:To evaluate the effects of Anshenning syrup on improving sleep and explore its mechanism.Research Methods1、The research of Anshenning syrup affecting the hypnotic time on mice by pentobarbital sodium in threshold dosageExperimental animals were divided into six groups:blank control group,extazolam control group,ethanol control group,anshenning syrup high,medium and low dose.Each group had twenty.Each drug group were given extazolam (0.13×10-3g/kg),ethanol (1.6g/kg),anshenning syrup (0.96g/kg、0.48g/kg、0.24g/kg).Blank control group were given the same volume of distilled water.Administratered by gavage of seven consecutive days.Given pentobarbital sodium (50mg/kg) by intraperitoneal injection after the last administration thirty minutes. Record the rats’sleep latent period and sleep time.2、The research of Anshenning syrup affecting the induence of sleep by pentobarbital sodium of subthreshold dosageExperimental animals were divided into six groups:blank control group,extazolam control group,ethanol control group,anshenning syrup high,medium and low dose.Each group had twenty.Each drug group were given extazolam (0.13X10-3g/kg),ethanol (1.6g/kg), anshenning syrup (0.96g/kg、0.48g/kg、0.24g/kg).Blank control group were given the same volume of distilled water. Administratered by gavage of seven consecutive days.Given pentobarbital sodium (50mg/kg) by intraperitoneal injection after the last administration thirty minutes.Record the number of sleep.3、The research of Anshenning syrup affecting sleep dose effect relationshipExperimental animals were divided into six groups:blank control group and five different dose groups of Anshenning syrup. Each group had twenty, Drug group administered with different doses of Anshenning syrup (0.12g/kg>0.24g/kg、0.48g/kg、0.96g/kg、1.92g/kg),Blank control group were given the same volume of distilled water. Administratered by gavage of seven consecutive days.Given pentobarbital sodium (50mg/kg) by intraperitoneal injection after the last administration thirty minutes. Record the rats’sleep latent period and sleep time.4、The research of Anshenning syrup affecting sleep time effect relationship:Experimental animals were divided into six groups:blank control group and five different delivery time groups of Anshenning syrup(three days^five days、seven days、nine days、eleven days), Each group had twenty. On the research of Anshenning syrup affecting sleep dose effect relationship basis, Drug group administered with Anshenning syrup (0.96g/kg), Blank control group were given the same volume of distilled water. Administratered by gavage of seven consecutive days.Given pentobarbital sodium (50mg/kg) by intraperitoneal injection after the last administration thirty minutes. Record the rats’sleep latent period and sleep time.5、The research of Anshenning syrup affecting the activities total sleep time and sleep phase of the mice.Experimental animals were divided into two groups, anshenning syrup group and ethanol control group, Each group had eight.Ethanol control group administered with ethanol (1.6g/kg), Anshenning control group administered with Anshenning syrup (0.96g/kg).Administratered by gavage of nine consecutive days. Taken the method of the cortical EEG recording for24h Observed Anshenning syrup affecting the activities total sleep time and sleep phase of the mice.6、The research of Anshenning syrup combined with the horse Xini (Flumazenil) of pentobarbital sodium in mice inducing by sleep timeExperimental animals were divided into four groups:blank control group, Anshenning syrup group, flumazenil group, Anshenning syrup+flumazenil group. Each group had twenty. Anshenning syrup group and Anshenning syrup+flumazenil group administered with Anshenning syrup (0.96g/kg).the other group administered with distilled water. By gavage for nine days. Blank control group and Anshenning syrup group were given pentobarbital sodium (50mg/kg) by intraperitoneal injection after the last administration thirty minutes. Anshenning syrup group and Anshenning syrup+flumazenil group were given flumazenil(0.1mg/ml,3.5mg/kg). Inject pentobarbital sod-ium(50mg/kg) after twenty minutes. Record the rats’sleep latent period and sleep time.Results1、Anshenning syrup on the threshold dose of pentobarbital sodium in mice induced by sleep time effectsThe experimental results show that, Compared with the blank control group,Anshenning syrup high dose group and medium dose group could significantly prolong the sleep time (P<0.01, P<0.05), low dose group had a tendency to extend the sleep time,but there was no significant difference(P>0.05). Anshenning syrup high dose group could significantly shortern sleep latency (P<0.05), medium dose group and low dose group had a tendency to shorten sleep latency, but there was no significant difference (P>0.05). Compared with the ethanol control group, Anshenning syrup high dose group could significantly prolong the sleep time and shorten sleep latency (P<0.05), medium dose group had a tendency to prolong the sleep time and shorten sleep latency, but there was no significant difference (P>0.05). Compared with the extazolam control group, Anshenning syrup high dose group had a tendency to prolong the sleep time and shorten sleep latency, but there was no statistically significant (P>0.5).2、Anshenning syrup on under threshold dose of pentobarbital sodium in mice induced by sleep incidence:The experimental results show that, With the blank control group Anshenning syrup high dose group and medium dose group could significantly increase the incidence of sleep (P<0.01,P<0.05),low dose group had a tendency to increase the incidence of sleep,but there was no statistically significant(P>0.05).3^Anshenning syrup affecting sleep dose-effect relationship:The experimental results show that, Anshenning syrup on improving sleep effectappears certain dose-effect relationship, in a dose range, along with the increase of dosage, increase the efficacy, the optimal effective dose of0.96g/kg.4、Anshenning syrup affecting sleep to time-effect relationship:The experimental results show that, Anshenning syrup improving sleep effect appears certain time-effect relation ship, within the scope of certain time, along with the delivery day increased, increase the efficacy.Primarily prolong pentobarbital sodium in mice induced by sleep duration (P<0.01) and shorten their sleep latency (P<0.01).Effective delivery time is three days, the best delivery time for nine days.5、Anshenning syrup activitied mice sleep duration and sleep phaseThe experimental results show that, in the sleep time, compared with the blank control group, in the circadian24h,Anshenning syrup group mices sleep time very significantly prolonged (P<0.01),awaking time very significantly shortened (P<0.01). In the sleep phase, compared with the blank control group, Non-rapid eye movements sleep(NREM) and fast eye movement sleep (REM) extend in varying degrees, in which the extension of the role of Non-rapid eye movements sleep(NREM) is particularly significant (P<0.01). Compared with the blank control group, in the sleep time,the echol mices sleep time prolonged effect were not obvious(P>0.5), In the sleep phase, Non-rapid eye movements sleep(NREM) and fast eye movement sleep (REM) prolonged effect were not obvious(P>0.5).6、Anshenning syrup combined with the horse Xini (Flumazenil) of pentobarbital sodium in mice induced by sleep time effectsThe experimental results show that,in comparison with blank control group,given alone Anshenning syrup,were prolonged pentobarbital sodium in mice induced by sleep duration and shortened the latency(P<0.01); Anshenning syrup compared with Anshenning syrup+Flumazenilwere prolonged pentobarbital sodium(P<0.05) in mice induced by sleep duration and shortened the latency(P<0.05); fluorine horse Xini can significantly blocked diazepam and Cirenshen effective part and sodium pentobarbital synergy effect of.Conclusion1、Anshenning syrup has a role in improving sleep.2、The optimal effective dose of Anshenning syrup improve sleep is0.96g/kg. the best delivery time for nine days.3、In the circadian24h,Anshenning syrup can prolong total sleep time in freele moving mice. In the sleep phase, Anshenning syrup can prolong Non-rapid eye movements sleep(NREM) and fast eye movement sleep (REM), where the prolonged action of Non-rapid eye movements sleep(NREM) was particularly notable.4、The role of Anshenning syrup on improving sleep maybe through adjusting benzodiazepine receptor.