| Objective:To investigate the relative effect of synthetic borneol in mice sleep time and sleep phaseMethods:1. Based on the tracing and analysis of mice EEG for 3 consecutive days, and the feature research on sleep time and sleep phase in mice.Select 20 ICR male mice, under the conditions of anesthesia and cerebral stereotactic, buried cortex and EMG electrodes.Use 16 physiological signal recorder for 3 consecutive days tracings cortical electroencephalogram (EEG) and electromyography and analyzed in order to accumulate during the day and night time awakening, sleep time, and when the cumulative percentage of each sleep phase (Wake%, NREM%, REM%) is observed indicators, study changes in the mice sleep time and sleep phase.2. Based on the tracing and analysis of mice EEG for 3 consecutive days, research on effect of synthetic borneol on the sleep phase and the sleep time of mice.Select 40 ICR male mice, Randomly divided into blank group and Drug Administration Group,20 rats in each group. Under the condition of anesthesia and brain stereotactic, buried cortex and EMG electrodes, restore seven days, and 6:00 pm every day during recovery, administration group dosed 0.4g/kg intragastric administration of 7 days, the control group were given the same volume of saline. Use 16 physiological signal recorder for 3 consecutive days tracings cortical electroencephalogram (EEG) and electromyography and analyzed in order to accumulate during the day and night time awakening, sleep time, and when the cumulative percentage of each sleep phase (Wake%, NREM%, REM%) is observed indicators, synthetic borneol research mice sleep duration and impact of the sleep phase changes.Results:1. Based on the tracing and analysis of mice EEG for 3 consecutive days, and the feature research on sleep time and sleep phase in mice.1.1 Based on the tracing and analysis of mice EEG for 3 consecutive days the feature research on sleep time of mice.The experimental results show that:First day monitoring period:day 12 hours 20 mice average cumulative time of awakening 312.37min; cumulative average sleep time was 390.53 min; 20 mice 12 hours at night cumulative average awakening time was 397.16 min; cumulative average sleep time was 320.19 min.Second day monitoring period:day 12 hours 20 mice average cumulative time of awakening 312.30min; cumulative average sleep time was 386.11 min; night 12 hours 20 mice average cumulative time of awakening 407.31min; cumulative average sleep time was 307.33 min.Third days monitoring period:day 12 hours 20 mice average cumulative time of awakening 315.69min; cumulative average sleep time was 391.60 min; night 12 hours 20 mice average cumulative time of awakening 409.01min; cumulative average sleep time was 317.33 min.During the three days to monitor, cumulative time of 20 mice awakening day and night of continuous and cumulative sleep time no significant difference (P> 0.05)1.2 Based on the tracing and analysis of mice EEG for 3 consecutive days the feature research on sleep phase of mice.The results display that:During the first day of monitoring:day 12 hours 20 mice sleep phase Wake percentage average of 44.93%; the percentage of NREM sleep phase with an average of 49.26%; relative to the average percentage of REM sleep was 5.42%; phase 12 hours during the night of 20 mice sleep Wake percentage average of 55.99%; the percentage of NREM sleep phase with an average of 40.03%; percentage of REM sleep phase average of 4.74%.During the second day of monitoring:day 12 hours 20 mice sleep phase Wake percentage average of 45.68%; the percentage of NREM sleep phase with an average of 48.26%; relative to the average percentage of REM sleep was 5.69%; phase 12 hours during the night of 20 mice sleep Wake percentage average of 58.26%; the percentage of NREM sleep phase with an average of 38.40%; percentage of REM sleep phase with an average of 4.70%.During the third day of monitoring:day 12 hours 20 mice sleep phase Wake percentage average of 45.17%; the percentage of NREM sleep phase with an average of 48.72%; relative to the average percentage of REM sleep was 5.91%; phase 12 hours during the night of 20 mice sleep Wake percentage average of 58.70%; the percentage of NREM sleep phase with an average of 36.94%; percentage of REM sleep phase average of 4.69%.Monitoring for 3 consecutive days, day and night when sleep phase Wake, sleep phase NREM, the percentage of no significant difference (P> 0.05) with REM sleep.2. Based on the tracing and analysis of mice EEG for 3 consecutive days, research on effect of synthetic borneol on the sleep phase and the sleep time of mice.2.1 Based on the tracing and analysis of mice EEG for 3 consecutive days, research on effect of synthetic borneol on the sleep time of mice.The experimental results show that:In the 3 day continuous monitoring, The drug group in mice after giving synthetic borneo:First days, compared with the control group, daytime, accumulation awakening time was significantly shorter (P<0.01), the cumulative sleep time was significantly increased (P<0.01); night, accumulation awakening time was significantly increased (P<0.01), the cumulative sleep time significantly reduced (P<0.01).Second days, compared with the control group, daytime, accumulation awakening time was significantly shorter (P<0.01), the cumulative sleep time was significantly increased (P<0.01); night, accumulation awakening time was significantly increased (P<0.01), the cumulative sleep time significantly reduced (P<0.01).Third days, compared with the control group, daytime, accumulation awakening time was significantly shorter (P<0.01), the cumulative sleep time was significantly increased (P<0.01); night, the cumulative time of awakening increased (P<0.05), the cumulative sleep time (P<0.05).Day 1 treatment group compared with the second day dosing group, during the day, the cumulative time of awakening was no significant difference (P> 0.05), the cumulative sleep time was no significant difference (P> 0.05); night, accumulation awakening No significant difference (P> 0.05); the cumulative sleep time was no significant difference (P> 0.05).Day 2 treatment group compared with the third day dosing group, during the day, the cumulative time of awakening was no significant difference (P> 0.05), the cumulative sleep time was no significant difference (P> 0.05); night, accumulation awakening No significant difference (P> 0.05); the cumulative sleep time was no significant difference (P> 0.05).2.2 Based on the tracing and analysis of mice EEG for 3 consecutive days, research on effect of synthetic borneol on the sleep phase of mice.In three consecutive days of monitoring, administered in mice after administering synthetic borneol:First days, compared with the control group, during the day, sleep phase Wake reduce the percentage to 34.81%(P<0.01), when the phase NREM sleep and REM sleep phase percentage increased to 57.76%(P<0.01, respectively),6.10%(P> 0.05); at night, sleep with Wake increase the percentage to 67.55%(P<0.01), when the phase NREM sleep and REM sleep phase percentage decreased to 30.24%(P<0.01),3.40%(P> 0.05)Second days, compared with the control group, during the day, sleep phase Wake reduce the percentage to 35.81%(P<0.01), when the phase NREM sleep and REM sleep phase percentage increased to 56.67%(P<0.01, respectively),6.18%(P> 0.05); at night, sleep with Wake increase the percentage to 67.50%(P<0.01), when the phase NREM sleep and REM sleep phase percentage decreased to 30.88%(P<0.01),3.00%(P> 0.05)Third days, compared with the control group, during the day, sleep phase Wake reduce the percentage to 37.26%(P<0.01), when the phase NREM sleep and REM sleep phase percentage increased to 55.62%(P<0.01, respectively),5.60%(P> 0.05); at night, sleep with Wake increase the percentage to 65.06%(P<0.01), when the phase NREM sleep and REM sleep phase percentage decreased to 33.43%(P<0.05),3.17%(P> 0.05)First days to the drug group compared with the second day dosing group, during the day, There was no significant difference in the percentage of sleep phase Wake (P> 0.05). There was no significant difference in the percentage of sleep phase NREM (P> 0.05). There was no significant difference in the percentage of sleep phase REM (P> 0.05). In the night, There was no significant difference in the percentage of sleep phase Wake (P> 0.05). There was no significant difference in the percentage of sleep phase NREM (P> 0.05). There was no significant difference in the percentage of sleep phase REM (P> 0.05).Second days to the drug group compared with the third day dosing group, during the day, There was no significant difference in the percentage of sleep phase Wake (P> 0.05). There was no significant difference in the percentage of sleep phase NREM (P> 0.05). There was no significant difference in the percentage of sleep phase REM (P> 0.05). In the night, There was no significant difference in the percentage of sleep phase Wake (P> 0.05). There was no significant difference in the percentage of sleep phase NREM (P> 0.05). There was no significant difference in the percentage of sleep phase REM (P>0.05).Conclusion:1. Based on the tracing and analysis of mice EEG for 3 consecutive days, and the feature research on sleep time and sleep phase in mice:Sleep time:during the day, the cumulative time of awakening 312.37min, cumulative sleep time was 390.53 min; night time was 397.16 min cumulative awakening cumulative sleep time 320.19min;Sleep phase:during the day, Wake, NREM, REM percentage was 45.26%, 47.56%,5.67%; night, Wake, NREM, REM percentage was 57.73%,39.12%, 4.71%. Tracings of mice during the phase when the sleep and sleep time was stable.2. Effects of Synthetic Borneol on sleep duration and sleep phases in mice for 3 consecutive days:During the day, the cumulative time of awakening were significantly shortened; cumulative sleep time were significantly prolonged; night, accumulation awakening time there was a significant prolongation:cumulative sleep time were significantly shortened. Phases during sleep, daytime, Wake percentage decreased, the percentage were increased NREM, REM percentage change was no significant difference; more than one hundred nights Wake percentage increased significantly to reduce the percentage of NREM, REM percentage the percentage change was no significant difference... |
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