Research Of Components In Radix Psuedostellariae Based On Fingerprint

Radix Pseudostellariae is authentic herbs of Fujian province, mainly produced in Fujian province, Jiangsu province, Guizhou province and other places, chemical composition in Radix Pseudostellariae are certain differences among different provinces planted. Radix Pseudostellariae containes in the chemical composition of a wide range, but its relationships between efficacy and chemical composition are relatively weak, systematic study of the subject of the chemical composition of traditional Chinese medicine and its efficacy, provides a theoretical basis correlation is perfect heterophylla quality evaluation method.At first, we collect samples from different herbs origin, to establish HPLC fingerprint and quantitative analysis of the total composition by liquid chromatography-mass spectrometry techniques base on the fingerprint, and then, to gain chromatographic separation parts for efficacy experiments, finally, we build effective relationships to explore the efficacy associated spectral components.Test the collected samples according to the pieces of Radix Pseudostellariae herbs and regulations in "the Chinese Pharmacopoeia", Identified from medicinal properties, physical and chemical identification, and aspects of TLC, as a result,15batches of Radix Pseudostellariae from different origin experimental results of moisture between4.54%～6.29%, total ash content of between2.12%～2.82%, water-soluble extract content between30.63%～35.65%, all are in line with Pharmacopoeia requirements.By examining the pretreatment and HPLC conditions, to determine ethyl acetate as the extraction solvent, to determine Soxhlet extraction method as extraction method; optional mobile phase acetonitrile-water (0.2%phosphoric acid) gradient, flow rate1.OmL/min, inject and analysis for90min,203nm as detection wavelength; evaluation samples of different batches Radix Pseudostellariae similarity, and comparing the total peak marked difference in the overall similarity of the same origin Pseudostellariae samples reached0.85or more, RSD of retention time of each common peak were less than1.0%, more similar peaks in total peak difference are4#,6#,7#,9#,10#,15#,17#-23#, Suggesting that these ingredients are present in different Radix Pseudostellariae samples. On the basis of HPLC fingerprint on by liquid chromatography-mass spectrometry, we finished components identification heterophylla. There contains amino acids between0-5minutes, the γ-aminobutyric acid quantification, tryptophan, phenylalanine, tyrosine, leucine, isoleucine6kinds of amino acids; mainly5-15minutes cyclic peptide compounds, the apparent molecular ion peak of law and literature data identified11kinds of cyclic peptides;15-30minutes mainly fatty compounds, quantitative linoleic acid, palmitic acid, two kinds of fatty acids. Wherein the cyclic peptide heterophylla Pesudostellarin A, Pesudostellarin B, Pesudostellarin F, Pesudostellarin G, Heterophyllin C, and various fatty acids exist among the different samples.