Notopterygium And Independent Living Herbs Chromatographic Fingerprint

Rhizoma et Radix Notopterygii is the dried rhizome and root of Notopterygium incisum Ting ex H.T.Chang or Notopterygium forbesii Boiss., Radix Angelicae Pubescentis is the dried root of Angelica pubescens Maxim.f.biserrata Shan et Yuan, which are widely used in clinic and also recorded in ChP as common Chinese traditional herb. Because their sources, efficacy and characteristics are similar, it is easy to confuse them in the market and clinical medication. 17 samples of Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis were collected from different habitats. The GC and HPLC fingerprints and content of essential oils of Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis were compared. The integrated quality assessment of Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis was developed.Essential oil and coumarins have been reporting as effective components. We determined the content of essential oil from Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis. The fingerprints of them were established by GC-FID. DB-1 capillary column (30 m×0.25 mm×0.25μm) was used, and the temperature of column was 50℃after injection then programmed at 8℃·min^-1 to 85℃for 12 min and at 4℃·min^-1 to 155^-1 for 18 min and at 12℃·min^-1 to 250℃for 5 min. The temperature of detector was 270℃. 17 batches of Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis were studied under this condition. There was evident difference between them. With the Cosine used as the measurement index, the Hierarchical cluster was applied to the GC fingerprints of Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis. 17 batches of Rhizoma et Radix Notopterygii were divided into three grades. Among them the grade I was commendatory and the grade II, III were general. Mutual pattern was established from 15 batches in the grade I by using the fingerprint chromatogram software required to use by the Chinese Pharmacopoeia Committee. Similarity calculations were studied by comparing the GC fingerprint chromatograms of Rhizoma et Radix Notopterygii and mutual pattern. The results of the commendatory should be over 0.85; 17 batches of Radix Angelicae Pubescentis were divided into four grades. Among them the grade I was commendatory and the grade II, III, IV were general. Mutual pattern was established from 11 batches in the grade I . Similarity calculations were studied by comparing the GC fingerprint chromatograms of Radix Angelicae Pubescentis and mutual pattern. The results of the commendatory should be over 0.85. Similarity calculations were studied by comparing the GC fingerprint chromatograms of Rhizoma et Radix Notopterygii and the mutual pattern of Radix Angelicae Pubescentis. The highest was 0.39. Similarity calculations were studied by comparing the GC fingerprint chromatograms of Radix Angelicae Pubescentis and the mutual pattern of Rhizoma et Radix Notopterygii. The highest was 0.52. The method of GC fingerprints can accurately identify Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis and the difference of content of essential oils can also identify them.To establish a method for the identification of Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis by HPLC fingerprints. The chromatographic condition included Diamonsil C₁₈ (250 mm×4.6 mm, 5μm) column with the mobile phase consisting of methanol- acetonitrile (1:1) (A) and 0.03% phosphoric acid solution (B) with gradient elution mode. The following gradient procedure was used: 0～30 min, 20%～46% A, 30～46 min, 46%～48% A, 46～48 min, 48%～72% A, 48～60 min, 72% A, 60～62 min, 72%～95% A, 62～70 min, 95% A. The detection was set at 320 nm. 17 batches of Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis were studied under this condition. There was evident difference between them. The Hierarchical cluster was applied to the HPLC fingerprints of Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis. 17 batches of Rhizoma et Radix Notopterygii were divided into three grades. Among them the grade I was commendatory and the grade II, III were general. Mutual pattern was established from 8 batches in the grade I . Similarity calculations were studied by comparing the HPLC fingerprint chromatograms of Rhizoma et Radix Notopterygii and mutual pattern. The results of the commendatory should be over 0.85; 17 batches of Radix Angelicae Pubescentis were divided into five grades. Among them the grade I was commendatory and the grade II, III, IV, V were general. Mutual pattern was established from 13 batches in the grade I . Similarity calculations were studied by comparing the HPLC fingerprint chromatograms of Radix Angelicae Pubescentis and mutual pattern. The results of the commendatory should be over 0.85. Similarity calculations were studied by comparing the HPLC fingerprint chromatograms of Rhizoma et Radix Notopterygii and the mutual pattern of Radix Angelicae Pubescentis. The highest was 0.22. Similarity calculations were studied by comparing the HPLC fingerprint chromatograms of Radix Angelicae Pubescentis and the mutual pattern of Rhizoma et Radix Notopterygii. The highest was 0.11. The method of HPLC fingerprints can effectively identify Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis. In this study Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis were assessed and identified by GC and HPLC fingerprints. The method was simple, rapid and reliable and can provide basis for the distinction and quality control of Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis.