Expression And Significance Of Annexin A1in Varying Cervical Disease And Analyse The Correlation With High Risk HPV

Objective:1To detect the expression of Annexin A1in normal cervicalepithelium、cervical intraepithelial neoplasia and cervical carcinoma anddiscuss the clinical significance.2To statistic the positive rate of high risk HPV infection in normalcervical epithelium、cervical intraepithelialneoplasia and cervical cancer.3To analyse the correlation of Annexin A1and high risk HPV in varyingcervical disease.Methods:Collect105clinical specimans which has complete clinical date.dividedthem into3groups.(30with normal cervical epithelium,42with CIN and33with cervical carcinoma).then divided the CIN group into3group(15withCINⅠ,13with CINⅡ,14with CIN Ⅲ). Detected the expression of AnnexinA1using immunohistochemical S-P methods. Statistic the positive rate of highrisk HPV infection in each group and analyse the correlation between AnnexinA1and high risk HPV.Results:1The expression of Annexin A1was localized in cell memebrane andcytosol. The Annexin A1expression was varying in different groups.Significant differences of Annexin A1expression were noted among thecontrol group、 CIN group and cervical carcinoma group (p<0.01). thepositive rate in the three groups was93.3%、74.1%、45.5%. Among the threeCIN groups.Positive rate of Annexin A1expression was86.7%、69.2%、57.1%in CINⅠ、CINⅡand CINⅢ group and the difference was statisticalsignificant.The positive rate decrease was in accordance with the increasedseverity of cervical atypical hyperplasia. These results revealed a negative correlation between Annexin A1expression and the severity of cervicaldiseases(r=-0.403).In addition, Annexin A1expression was closely related tothe tissue classification of cervical carcinoma, The Annexin A1expressionwas obvious decresase in the specimens with lower tissue differentiation ofcervical carcinoma. No significant differences was observed between AnnexinA1expression and the clinical stage of patients with cervical carcinoma andmetastasis.2The positive rates of high risk HPV infection in normal cervicalepithelium group,CIN grouo and cervical carcinoma group were23.3%,81.0%and100%, there were significant differences among the three groups(p<0.01).Among the three CIN groups, the positive rate of high risk HPV infectionincreased from66.7%to84.6%and92.9%, however, there were nosignificant differences among the three groups(p>0.05). The positive rates ofhigh risk HPV infection increased in accordance with the increased severity ofcervical disease.High risk HPV infection positively correlated with theseverity of the cervical disease(r=0.644).3Correlation analysis showed that Annexin A1expression negativelycorrelated the high risk HPV expression in varying cervical disease.(p=0.001, r=-0.338).Conclusion:1In varying cervical disease, the positive rate of Annexin A1expressiondecreased with the increased degrees of cervical diseases, it negativelycorrelated with the degrees of cervical diseases.That suggested Annexin A1play a role in the initiation and progression of cervical carcinoma.2The expression of Annexin A1negatively correlated with the tissueclassification of cervical carcinoma, That suggested Annexin A1could be abiological marker for evaluating the degree of cervical carcinoma.3In cervical diseases, the positive rate of high risk HPV infectionincreased with the increased degrees of cervical diseases, it positivelycorrelated with the degrees of cervical diseases.4Annexin A1expression negatively correlated with the high risk HPV infection in the cervical diseases, they had close relationship in the initiationand progression of CIN and cervical carcinoma.