Analysis Of T,B Lymphocytes And NK Cells In Peripheral Blood With High-risk HPV Infection

Objective:In this study,by detecting the percentage of CD3~+T cells,CD3~+CD4~+T cells,CD3~+CD8~+T cells,CD3~-CD56~+(Natural Killer cell,NK cells),CD3~-CD19~+B cells and the ratio of CD4/CD8 and analyzing the changes in peripheral blood of cervical high-risk Human papillomavirus(HPV)infection,to comprehend the systemic immune function in patients of cervical high-risk HPV infection,and to provide an objective basis for immune intervention and prognosis in clinical high-risk HPV infection.Methods:Gynecologic outpatients and ward patients were selected in our hospital for screening cervical cancer from November 2016 to November 2017,134cases with high-risk HPV-DNA positive were collected as case group,including 31 cases of Subclinical papillomavirus infection(SPI),24 cases of cervical intraepithelial neoplasia grade I(CIN I),26 cases of cervical intraepithelial neoplasia grade II(CIN II),29 cases of cervical intraepithelial neoplasia grade III(CIN III)and 24 cases of squamous cervical carcinoma(SCC).Meanwhile,30 cases of healthy people with high risk HPV-DNA negative and ThinPrep-cytology(TCT)normal were selected as Normal control group(NC group).Detecting the percentage of patients'peripheral blood lymphocyte by flow cytometry,and analyzing the differences of the expression levels for CD3~+T cells,CD3~+CD4~+T cells,CD3~+CD8~+T cells,CD3~-CD56~+NK cells,CD3~-CD19~+B cells and the ratio of CD4/CD8 among the groups.Then,Statistical software SPSS 22.0 was used to analyze all the datas,the measurement data was descriptioned with mean±SD,the comparison between the five groups was based on the analysis of variance(ANOVA).Count data were compared by chi square test;P<0.05 indicates that the difference was statistically significant.Results:1 Age were compared in each group.Based on the comparison among the six groups in age,results showed that there were statistically significant differences in age among the six groups(P<0.05).After that,the multiple comparisons for the population mean of the age among the six groups,the result shows that there was no statistically significant difference between the control group and SPI group.The same happened in group of CIN II and CIN III(P>0.05).Nevertheless,the difference between the rest of the other was statistically significant(P<0.05).2 The infection of high-risk HPV16/18 types and other types were compared in the group of SPI,CIN I,CIN II,CIN III and SCC.In the group of SPI,the number of HPV16/18 types was 11(35.48%),the number of other types was 20(64.52%);in the group of CIN I,the number of HPV16/18 types was 10(41.67%),the number of other types was 1(58.33%);in the group of CIN II,the number of HPV16/18 types was 16(61.54%),the number of other types was 10(38.46%);in the group of CIN III,the number of HPV16/18 types was 18(62.07%),the number of other types was 11(37.93%);in the group of SCC,the number of HPV16/18 types was 17(70.83%),the number of other types was 7(29.17%).Constituent ratio of high-risk HPV16/18 and other types were compared among the five groups,the results shows that there were significant differences(?~2=9.83,P<0.05).3 Comparison of the expression level of peripheral blood lymphocyte subsets in each groupIn this study,there was no significant difference among the three groups of control,SPI and CINI(P>0.05).Meanwhile,there was no significant difference in group of CIN II and CIN III(P>0.05).Compared with subclinical infection group and control group,CD3~+CD4~+T cells,CD3~-56~+NK cells in groups of CIN?,CIN?and SCC were decreased,CD3~+CD8~+T cells were increased,the ratio of CD4/CD8 decreased,the differences were significant(P<0.05).With the progress of the pathological staging,the level of CD3~+CD4~+T cells,CD3~-56~+NK cells were decreased gradually,CD3~+CD8~+T cells were increased gradually,the ratio of CD4/CD8 in group of SCC was significant lower than group of CIN I,and the inversion was observed,the difference was statistically significant(P<0.05),while there was no significant differences in CD3~+T cells and CD3~-CD19~+B cells among the four groups(P>0.05).4 Comparison of the expression level of peripheral blood lymphocyte subsets in CIN II,CIN III and SCC groups with different high-risk types of HPV infectionThe percentage of CD3~+T cells,CD3~+CD4~+T cells,CD3~+CD8~+T cells,CD3~-CD56~+NK cells,CD3~-CD19~+B cells and the ratio of CD4/CD8 were compared between the type of HPV16/18 and other types among the group of CIN II,CIN III and SCC,the result shows that there was no significant difference in the cell changes(P>0.05).Conclusion:1 Patients with high grade cervical lesions and squamous cervical carcinoma of the cervix were mainly infected by type of HPV16/18,patients with high risk HPV subclinical infection and low grade cervical lesions are mainly infected by other types.With the progress of cervical pathological staging,the proportion of HPV16/18 types increased.Therefore,the type of HPV16/18 infection is an important factor leading to cervical precancerous lesion and cervical cancer.2 There are different degrees of cellular immunity and innate immunity decreased in patients with high-grade cervical lesions and cervical cancer.The changes of lymphocyte subsets were related to pathological staging of cervix in patients with high-risk HPV infection,and with the progress of cervical pathological staging,the migration of lymphocyte subsets in the patients was becoming more and more significant,however,in the same pathological stage,changes of lymphocyte subsets was not related to the type of high-risk HPV infection.3 Patients with high-risk HPV subclinical infection and low grade cervical lesions,compared with the control group,there was no significant change in lymphocyte subsets.This indicates that the systemic immune function for patients of subclinical infection and low grade cervical lesions were basically normal.4 Detecting the changes of all kinds of lymphocyte subsets can be used to further explore the immune mechanism of high-risk HPV infection of cervix,and provide objective basis for the immune intervention and prognosis of clinical high-risk HPV infection.