A Clinical Study Of Changes Of Resistin、C-Reactive Protein Level And Insulin Resistance In Gestational Diabetes Mellitus

Objective:By detecting points blood glucose (FPG) of Glucose tolerance test (OGTT) and Points of insulin (FINS) values of Synchronize insulin release test in Pregnant women among the normal glucose tolerance (NGT) group, impaired glucose tolerance (IGT) group and gestational diabetes mellitus (GDM) group, we compared the levels of maternal plasma Resistin and C-reactive protein (CRP) in three groups and analyzed their correlations on the above indicators, in order to explore the relationship among plasma Resistin、CRP and Insulin Resistance (IR) in Gestational diabetes mellitus pregnant women.Methods:We choose metaphase and late pregnancy women of Periodic inspection in affiliated hospital of Guilin medical college from April2010to December2012,and do the50gram glucose screening test in24-28pregnancy weeks. Pregnancy women must do the oral75gram oral glucose tolerance test in a week when their results≥7.8mmol/L and<11.1mmol/L. According to the diagnostic criteria of the seventh edition of the Gynecology and obstetrics, edited by Le Jie and published by people’s medical publishing house, the subjects are divided into three groups:NGT Group (60cases), the IGT Group (60cases), GDM Group (60cases).We detected points blood glucose (FPG) of Glucose tolerance test (OGTT) and Points of insulin (FINS) values of Synchronize insulin release test、the levels of Resistin and C-reactive protein (CRP) in fasting plasma in three groups. We used the hexokinase method to determine glucose, Electrochemiluminescence immunoassay to determine insulin, Enzyme-linked immunosorbent assay (ELISA) to determine the levels of plasma Resistin, Immune transmission turbidimetry to determine the level of CRP. While we took advantage of steady-state model of insulin resistance index (HOMA-IRI) to evaluate the degree of insulin resistance, and compare groups differences in degree of insulin resistance. SPSS statistics software was used. Continuity measurement information are expressed in mean±standard deviation. Compared with indicators of groups using analysis of variance.The statistically significant level was P<0.05.We used simple correlation analysis to study the relationship of Resisting、C-reactive protein (CRP) and insulin resistance (IR).Results:(1)Comparison among three groups in fasting blood glucose、1hour postprandial blood glucose、2hours postprandial blood glucose、3hours postprandial blood glucose:GDM group>IGT group>NGT group, differences are statistically significant(P<0.05).(2)Fasting insulin of GDM group is higher than IGT group and NGT group in three groups, differences are statistically significant(P<0.01),but Comparison with IGT group and NGT group,differences are not statistically significant(P>0.05).(3)Comparison of HOMA-IRI of three groups:GDM group>IGT group>NGT group, GDM group is higher than IGT group and NGT group (P<0.01),but differences are not statistically significant between IGT group and NGT group.(4)Plasma Resistin and C-reactive protein in GDM group are both IGT group (P<0.01) and NGT group (P<0.01),but differences are not statistically significant between IGT group and NGT group.(5)Correlation analysis showed that Plasma Resistin and C-reactive protein of GDM group were positive correlations with BMI、FPG、 FINS、HOMA-IRI in late pregnancy. Positive correlation was also found between Plasma Resistin and C-reactive protein.Conclusions:(1) Patients with GDM have insulin resistance significantly, this is similar to type II diabetes.(2)Plasma Resistin levels are significantly higher in patients with GDM, and Resistin level are positive related with the level of insulin and HOMA-IRI.It prompt that Resistin may be involved in the formation of IR in patients with GDM.(3)The level of C-reactive protein had risen in Patients with GDM. The level of C-reactive protein is related with IR because of that the level of C-reactive protein has both positive correlation between the level of insulin and HOMA-IRI.(4)Resistin has positive correlation with C-reactive protein in patients with GDM, supplementing each other.